测序新技术Ion Torrent PGM及其法医学应用  

The new sequencing technology-Ion Torrent PGM and its forensic application

作　　者：刘宇轩[1,2] 张文琼[1] 黄代新[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]随县公安局刑警大队,湖北随州441300

出　　处：《中国法医学杂志》2015年第1期52-55,共4页Chinese Journal of Forensic Medicine

摘　　要：目前,基因测序技术发展迅速,继Roche公司的454系统、Illumina公司的Hi Seq 2000系统和AB公司的SOLi D系统之后,Life Technologies公司于2010年推出了Ion Torrent Personal Genome Machine(PGM)测序仪。Ion Torrent是基于半导体芯片的测序技术,不属于核酸标记、荧光检测的生化技术。与其他测序技术相比,操作简便,费用较低,具有强大的扩展性。本文就Ion Torrent PGM的特点及其在法医学中的应用进行简要介绍。Gene sequencing technology has been rapidly developed in recent years. Following Roche＇ s 454, Illumina＇ s HiSeq 2000 and the AB＇ s SOLID systems, Life Technologies launched the Ion Torrent Personal Genome Machine （PGM） sequencer in 2010. Ion Torrent is a semiconductor chip based sequencing technology, and does not belong to biochemical techniques like nucleic acid labeling and fluorescence detection. Compared with other sequencing technologies, Ion Torrent is easier to operate, more economical, and is of a strong scalability. This paper is aimed to make a brief review on the characteristics of Ion Torrent PGM and its application in forensic science.

关 键 词：法医物证学 NGS ION Torrent 法医学应用 

分 类 号：D919[医药卫生—法医学]