清热化湿舒胃方及其寒热配伍对幽门螺旋杆菌的体外抑菌实验  

In vitro bacteriostatic activity of Qingre Huashi Shuwei formula and its hot-cold drug combinations against Helicobacter pylori

作　　者：陈智龙[1] 吕永慧[1] 王玎[2] 涂星[3] 

机构地区：[1]广州中医药大学附属广州市中医医院脾胃科,广东省广州市510130 [2]广州中医药大学中药学院,广东省广州市510006 [3]广州中医药大学第一附属医院临床药学部,广东省广州市510405

出　　处：《世界华人消化杂志》2015年第6期949-953,共5页World Chinese Journal of Digestology

基　　金：国家科技部"十一五"国家科技支撑计划;No.2007BAI20B094~~

摘　　要：目的:探讨清热化湿舒胃方及其寒热配伍对幽门螺旋杆菌(Helicobacter pylori,H.pylori)的体外抑制作用.方法:运用琼脂扩散法、液体稀释法考察清热化湿舒胃方及其寒热配伍对H.pylori的最低抑菌浓度(minimal inhibitory concentration,MIC)与最低杀菌浓度(minimum bactericidal concentration,MBC).结果:清热化湿舒胃方组、热性配伍组、寒性配伍组和抗生素组的抑菌环直径分别为14.30 mm±1.12 mm、3.13 mm±0.82 mm、13.93 mm±0.95 mm、23.25 mm±1.33 mm,清热化湿舒胃方的MIC为7.81 mg/m L,MBC为15.63 mg/m L.结论:清热化湿舒胃方及其寒性配伍组对H.p y l o r i具有较好的抑制作用,且清热化湿舒胃方对H.pylori的抑制作用可能是由寒性药物黄芩和蒲公英实现的.AIM： To compare the in vitro bacteriostatic activity of Qingre Huashi Shuwei formula and its hot-cold drug combinations against Helicobacter pylori （H. pylori）. METHODS： The minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of Qingre Huashi Shuwei formula and its hot-cold drug combinations against H. pylori were determined using agar diffusion method and dilution method. RESULTS： The bacterostasis circle diameters of Qingre Huashi Shuwei formula, hot drug group, cold drug group, and antibiotics were 14.30 mm ± 1.12 mm, 3.13 mm ±0.82 mm, 13.93 mm ± 0.95 mm, and 23.25 mm ± 1.33 ram, respectively. The MIC of Qingre Huashi Shuwei formula was 7.81 mg/mL, and the MBC was 15.63 mg/mL. CONCLUSION： Qingre Huashi Shuwei formula and its cold drug combination perform better in terms of in vitro bacteriostatic activity against H. pylori, and the bacteriostatic activity of Qingre Huashi Shuwei formula against H. pylori mostly comes from its cold drugs.

关 键 词：清热化湿舒胃方 幽门螺旋杆菌 抑菌作用 最低抑菌浓度 最低杀菌浓度 

分 类 号：R285[医药卫生—中药学]