采用UPLC/Q-TOF MS与NMR代谢组学技术研究生长年限对远志药材质量的影响  

UPLC/Q-TOF MS and NMR plant metabolomics approach in studying the effect of growth year on the quality of Polygala tenuifolia

作　　者：薛英[1,2] 李晓伟[1,2] 李震宇[1] 曾祖平[3] 张福生[1] 李爱平[1,2] 秦雪梅[1] 彭冰[3] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]北京市中医研究所,首都医科大学附属北京中医医院,北京100010

出　　处：《药学学报》2015年第3期340-347,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31100244);山西省科学技术发展计划资助项目(20140313010-1);国家科技支撑计划资助项目(2011BAI07B05);山西省创新团队资助项目(2013131015)

摘　　要：生长年限是影响远志药材质量的重要因素之一。本文对陕西合阳1～3年生栽培远志中的初级和次级代谢物进行了比较。首先对上述样品分别进行超高效液相色谱-四极杆-飞行时间质谱和核磁共振分析,采用主成分分析等多种统计分析方法,找出差异代谢物并对其含量及相关性进行分析。结果显示,1年与2、3年生远志可明显区分,而2年与3年生远志则区分不明显。随着远志生长年限的延长,初级代谢物中蔗糖、果糖、胆碱的含量减少,而甘氨酸、棉籽糖的含量则增加;次级代谢物中皂苷类成分（onjisaponin Fg、polygalasaponin XXVIII、polygalasaponin XXXII）的含量增加,而口山酮（polygalaxanthone III）及部分低聚糖酯（tenuifoliose A、tenuifoliose C、tenuifoliose C2、tenuifoliose H）的含量则减少。生长年限对远志药材质量会产生较大影响,而商品远志药材的现有生长年限有一定的科学依据。本研究为现有中药材质量评价方法提供了一种新的思路。Growth year is one of the important factors for the quality of Polygala tenuifolia. In this study, primary metabolites and secondary metabolites were compared in 1, 2 and 3 years old P. tenuifolia cultivated in Shaanxi Heyang. The samples were subjected to ultra-high performance liquid chromatography （UPLC）-quadrupole time-of-flight mass spectrometry （Q-TOF MS） and nuclear magnetic resonance （NMR） analysis, and the obtained data were analyzed using principal component analysis （PCA） and other statistical analysis methods. In addition, content and correlation of different metabolites were also calculated. The results showed no significance between main component contents in 2 year-old and 3 year-old P. Tenuifolia,but 1 year-old was statistically different. The contents of primary metabolites, such as fructose, sucrose, and choline increased as time goes on, while glycine and raffinose decreased. The contents of secondary metabolites, such as onjisaponin Fg, polygalasaponin XXVⅢ, polygalasaponin XXXⅡ increased, while polygalaxanthone Ⅲ and parts of oligosaccharide multi-ester including tenuifoliose A, tenuifoliose C, tenuifoliose C2 and tenuifoliose H decreased with the extension of the growth years. Growth years has important impact on the quality of P. tenuifolia and the existing growing years of commodity P tenuifolia have its scientific evidence. This study supplied a new method for the quality evaluation of Chinese medicinal materials.

关 键 词：远志 UPLC/Q-TOF MS NMR 代谢组学 生长年限 

分 类 号：R917[医药卫生—药物分析学]