丁苯酞对大鼠心肌梗死周边区交感神经重构的影响  

Effect of dl-3n-butylphthalide on sympathetic remodeling at infarct border zone in rats with myocardial infarction

作　　者：李俊[1] 黄从新[1] 

机构地区：[1]武汉大学人民医院心血管内科,430060

出　　处：《中华老年医学杂志》2015年第3期308-311,共4页Chinese Journal of Geriatrics

摘　　要：目的 探讨丁苯酞（NBP）对大鼠心肌梗死（心梗）后心功能和交感神经重构的影响.方法 成年雄性SD大鼠50只随机分为假手术组（10只）、心梗组（20只）和丁苯酞组（20只）.采用结扎大鼠心脏冠状动脉前降支制备心梗模型,给予丁苯酞灌胃治疗,1周后行血流动力学检测心功能,苏木精伊红（HE）染色和马松（Masson）染色,免疫组化检测心梗周边区域生长相关蛋白-43（GAP43）及酪氨酸羟化酶（TH）阳性神经纤维密度和分布,蛋白免疫印迹（Western blot）法检测核因子κB（NF-κB）信号通路的影响. 结果在实验观察期间,心梗组死亡8只,其中24 h内死亡2只.丁苯酞组死亡7只,其中24 h死亡3只.假手术组、心梗组、丁苯酞组左心室舒张末期压（LVEDP）分别为（7.81±2.46）mmHg（1 mmHg=0.133 kPa）、（18.93±3.17）mmHg、（16.56±4.82） mmHg,左心室收缩末期压（LVESP）分别为（136.44±13.62）mmHg、（103.32±10.59） mmHg、（112.83±16.97）mmHg,心梗组与丁苯酞组心室收缩时室内压最大上升速率（＋dP/dt）、左心室舒张时室内压最大下降速率（-dP/dt）比较差异无统计学意义（P＞0.05）.与假手术组比较,心梗组梗死周边区胶原容积分数增加（P＜0.05）,丁苯酞组较心梗组降低（P＜0.05）.心梗组梗死周边区GAP43和TH阳性的交感神经纤维密度增加,丁苯酞治疗后GAP43和TH阳性的交感神经纤维密度降低,假手术组、心梗组、丁苯酞组GAP43阳性神经密度、TH阳性神经密度比较,差异有统计学意义（均P＜0.05）.丁苯酞可降低p-IKBα的表达,增加IKBα蛋白表达. 结论 丁苯酞可能通过抑制NF-fB信号通路的活性改善心梗后交感神经重构.Objective To investigate the effect of dl-3n-Butylphthalide （NBP） on cardiac function and sympathetic remodeling in rats with myocardial infarction （MI）.Methods Totally 50 male SD rats were randomly divided into 3 groups：Sham-operated （sham） group （n 10）,myocardial infraction （MI） group （n=20） and MI with NBP group （n=20）.A rat myocardial infarction model was established by ligating anterior descending branch of coronary artery.Rats in MI with NBP group were received the gavage treatment with NBP.Cardiac function was evaluated by hemodynamic detection at 7 day post-MI.Cardiac tissues were stained with hematoxylin and eosin as well as Masson trichrome.The distribution and density of growth associated protein-43-（GAP-43） and tyrosine hydroxytase-（TH） positive nerve at infarction border zone were detected by immunohistochemistry.The activation of nuclear factor-κB （NF-κB） signaling pathway was detected by western blot analysis.Results 8 rats died in MI group,in which 2 rats died within 24h.7 rats died in MI with NBP group,and in which 3 rats died within 24 h.The left ventricular end-diastolic pressure （LVEDP） and left ventricular end-systolic pressure （LVESP） were （7.81 ± 2.46） mmHg （1 mmHg=0.133 kPa） and （103.32±10.59） mmHg,（18.93±3.17） mmHg and （136.44±13.62） mmHg,（16.56±4.82） mmHg and （112.83±16.97） mmHg in sham group,MI group and MI with NBP group respectively.There were no significant differences in ＋ dP/dt and-dP/dt between MI group and MI with NBP group （both P〉0.05）.Compared with sham group,the collagen volume fraction at infarct border zone was increased in MI group （P〈0.05）,but was reduced in MI with NBP group as compared to the MI group （P〈0.05）.The densities of GAP-43-and TH-positive nerves at infarct border zone were increased in MI group as compared with sham group,and reduced in MI with NBP group as compared with MI group （both P〈0.05）.NBP could reduced p IKBα protein expression and increas

关 键 词：心肌梗死 芹菜素 副交感神经系统 

分 类 号：R542.22[医药卫生—心血管疾病]