高粱C_4型pepc基因转入大豆可改善大豆光合特性  

Sorghum C_4-Specific pepc Gene Transformed into Soybean Can Improve the Photosynthetic Characteristics of Soybean

作　　者：张艳[1,2,3] 满为群[4] 南相日 李柱刚[4] 

机构地区：[1]黑龙江省农业科学院博士后工作站,哈尔滨150086 [2]东北林业大学博士后流动站,哈尔滨150040 [3]黑龙江省教育学院理科教育研培部,哈尔滨150080 [4]黑龙江省农业科学院大豆研究所,哈尔滨150086 [5]生物技术研究所,哈尔滨150086

出　　处：《分子植物育种》2015年第2期294-300,共7页Molecular Plant Breeding

基　　金：国家转基因专项转基因生物新品种培育项目(2011ZX08004-005)资助

摘　　要：改善作物的光合性能能够有效突破作物的产量潜力,目前国内关于转高粱C4型pepc基因大豆的研究尚未见报道。本研究以东北地区主栽大豆品种‘黑农41’为材料,通过农杆菌介导法将高粱C4型全长pepc基因转入‘黑农41’,获得了转基因植株。通过对转基因植株T0代和T1代进行了分子生物学检测、农艺性状调查和生理指标检测,结果表明目的基因已整合到大豆基因组中,T0代和T1代植株的净光合速率、气孔导度和蒸腾速率均有不同程度的提高,而胞间CO2浓度降低,其中T0代和T1代植株的净光合速率分别比对照提高了37.4%和20.7%,在T1代净光合速率增幅最大的T1-3-3,单株产量增加也最多,说明pepc基因的导入改善了转基因植株的光合特性,进而使转基因大豆产量提高。本研究可为高光效转基因大豆新品种的选育提供重要素材。As known,improving crop photosynthetic performance can effectively break crop yield potential,however,the study on transferring sorghum C4-specific gene into soybean did not yet report so far in China.In this research we transformed sorghum C4-specific full-length pepc gene into soybean line‘Heinong41＇ by Agrobacterium-mediated transferring method to obtain transgenic soybean lines PCR-Southern and Southern blot analysis indicated that the pepc gene should be indeed integrated into genome of soybean line‘Heinong41＇.Photosynthetic physiological analysis showed that net photosynthetic rate,stomatal conductivity and transpiration rate of most T0,T1 transgenic soybean lines were significant improved compared to the control of non-transformed plant,whereas intercellular CO2 concentration was decreased.The net photosynthesis rates of T0,T1 transgenic soybean lines increased by 37.4% and 20.7% than that of non-transformed control.The transgenic soybean line T1-3-3 had the highest net photosynthetic rate and production per plant.It indicated that the photosynthetic characteristics of the transgenic soybean lines harbouring pepc gene should have been improved to some extent as compared with their controls.The study might provide an important germplasm in the breeding program for high photosynthetic efficiency of the transgenic soybean.

关 键 词：大豆 磷酸烯醇式丙酮酸羧化酶(PEPC) C4型pepc基因 光合速率 

分 类 号：S565.1[农业科学—作物学]