利用ISSR分子标记分析29个风信子品种的遗传多样性  

Genetic Diversity of 29 Hyacinth Germplasm Resources Revealed by Using ISSR Markers

作　　者：胡凤荣[1] 胡月苗 王斐[1] 任翠[1] 

机构地区：[1]南京林业大学风景园林学院,南京210037

出　　处：《分子植物育种》2015年第2期379-385,共7页Molecular Plant Breeding

基　　金：江苏省高校优势学科建设工程资助项目(PAPD)资助

摘　　要：现代栽培的所有园艺风信子都是由原种风信子（Hyacinth orientalis）培育驯化而来,其遗传基础相对较窄,但经过几个世纪栽培选育,已经发生很大变化。为研究风信子品种之间的遗传关系,本研究利用12条引物对29个风信子品种进行ISSR分子标记研究,扩增获得109条谱带,多态性条带有103条,占条带总数的94.5%,表明风信子具有较高的遗传多样性。29个风信子品种的遗传距离范围为0.018 5~0.820 1,平均遗传距离为0.449 8。其中‘Atlantic’与‘Gipsy Queen’遗传距离最大为0.820 1,亲缘关系最远;‘Blue star’与‘Delf Blue’遗传距离最小,两品种之间相似度较大,亲缘关系最近。使用软件NTSYS-pc（2.10e版）获得UPGMA聚类树形图,以相似系数0.635为阈值,29个风信子品种可聚为两大类。聚类分析发现相同色系的品种几乎聚为一类,说明同色系风信子品种亲缘关系较近。因此,在杂交育种时可选择不同花色的品种作亲本,进行新品种的选育。All modern horticultural hyacinths are domesticated by the protospecies-Hyacinth orientalis.Though the protospecies have relatively narrow genetic basis,they have had great changes due to cultivation and breeding for centuries.In order to study the genetic relationship between varieties of hyacinth,12 primers of ISSR molecular markers were used to analyze 29 species of Hyacinth which generated 109 bands,including 103 polymorphism bands account for 94.5% of the total.Each pair of primers can amplify nine bands in average.The genetic distances among 29 Hyacinths orientalis were from 0.018 5 to 0.820 1 with the average of 0.449 8.There is the longest genetic distance of 0.820 1 between‘Atlantic＇and‘Gipsy Queen＇.Whereas between‘Blue star＇and‘Delf Blue＇being the shortest genetic distance.NTSYS-PC（Version 2.10e） was applied to UPGMA clustering analysis to construct the phylogenetic tree.By fixing the value of 0.635 as the threshold of similarity coefficient,29 species of hyacinths can be divided into two broad categories.The results showed that the varieties with the same color almost were divided into the same category,which indicated that the Hyacinth group has certain relevance with its color.

关 键 词：风信子 ISSR 遗传多样性 亲缘关系 

分 类 号：S682.29[农业科学—观赏园艺]