腹腔镜犬胆道梗阻模型研究  

Experimental study on canine biliary obstruction using laparoscopic technique

作　　者：薛飞[1,2,3] 吕毅[1,2,3] 虢宏昌 马锋[2,3] 陆建文[1,2,3] 李建鹏[1,2,3] 白纪刚[1,2,3] 刘亚雄[4,5] 

机构地区：[1]西安交通大学第一附属医院肝胆外科,陕西西安710061 [2]陕西省再生医学与外科工程技术研究中心,陕西西安710061 [3]西安交通大学先进外科技术与工程研究所,陕西西安710061 [4]西安工程大学机电工程学院,陕西西安710048 [5]西安交通大学机械制造系统工程国家重点实验室,陕西西安710049

出　　处：《中国普通外科杂志》2015年第2期211-215,共5页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(51275387);国家自然科学基金科学仪器专项基金资助项目(81127005)

摘　　要：目的:探讨应用腹腔镜微创技术制作胆道梗阻模型的动物模型的可行性。方法:用6只杂种犬进行腹腔镜下胆总管结扎手术,记录手术及术后情况,检测术前与术后(3、7、10 d)白细胞(WBC)、肝功能指标、C反应蛋白(C-RP)、血清降钙素原(PCT)水平,术后10 d观测胆道扩张情况及肝脏病理学改变。结果:总手术时间、麻醉苏醒时间、首次进食与排便时间分别为(39.17±3.4)min、(59.17±12.8)min、(3.5±0.63)h、1 d。WBC水平在术后3 d明显升高(P<0.05),但随后快速恢复正常(P>0.05);术后C-RP与PCT保持正常水平(均P>0.05);术后转氨酶水平先升后降,但均明显高于术前(均P<0.05);胆红素水平呈持续升高(均P<0.05)。术后10 d,肉眼可见胆总管扩张明显,病理学显示微胆管扩张。结论:利用腹腔镜技术建立犬胆道梗阻模型简便、微创、可行,该造模方法为今后的实验研究提供了便利。Objective： To investigate the feasibility of using laparoscopic minimally invasive technique to create animal model of biliary occlusion.Methods： Six mongrel dogs underwent laparoscopic common bile duct（CBD） ligation. The operative and postoperative variables were recorded, the preoperative and postoperative（day 3, 7 and 10） levels of white blood cells, liver function indexes, C-reactive protein（C-RP） and procalcitonin（PCT） were determined, and the CBD dilation and liver pathological changes were observed on postoperative day 10.Results： The overall operative time, time to recovery from anesthesia, time to first food intake and defecation was（39.17±3.4） min,（59.17±12.8） min,（3.5±0.63） h and 1 d, respectively. The WBC level was significantly elevated at postoperative day 3（P〈0.05）, but soon recovered（P〉0.05）; the postoperative C-RP and PCT levels remained normal（all P〉0.05）; the postoperative transaminase levels were initially increased and then decreased slowly, but all were significantly higher than those of preoperative levels（all P〈0.05）; the postoperative bilirubin levels showed continuous increase（all P〈0.05）. Obvious CBD dilation was seen macroscopically and pathological examination showed marked dilation of the bile capillaries.Conclusion： Establishment of canine model of biliary occlusion using laparoscopic technique is simple, minimally invasive and feasible, and this method of model creation may facilitate future experimental studies.

关 键 词：胆汁淤积 腹腔镜 模型 动物 

分 类 号：R657.4[医药卫生—外科学]