Cisplatin-loaded Poly(L-glutamic acid)-g-Methoxy Poly(ethylene glycol) Nanoparticles as a Potential Chemotherapeutic Agent against Osteosarcoma  

作　　者：Yi-fei Li Hai-yang Yu Hai Sun 刘建国 汤朝晖 Dan Wang Lian-you Yu Xue-si Chen 

机构地区：[1]Departemnt of Orthopaedics,the Norman Bethune First Hospital of Jilin University [2]Jilin Central Hospital [3]Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences [4]Basic Medical College,Beihua University [5]The Hospital of Jilin Chemical Industry Company

出　　处：《Chinese Journal of Polymer Science》2015年第5期763-771,共9页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51373168,51233004,21104076,51321062 and 51390484);Ministry of Science and Technology of China(International Cooperation and Communication Program 2011DFR51090);the Program of Scientific Development of Jilin Province(Nos.20130206066GX,20130727050YY and 20130521011JH)

摘　　要：Herein, cisplatin-loaded poly（L-glutamic acid）-g-methoxy poly（ethylene glycol） nanoparticles were evaluated as a potential chemotherapeutic agent against osteosarcoma by using alone or with an i RGD（internalizing RGD, CRGDKDPDC）. The release rate of platinum from the cisplatin-loaded nanoparticles CDDP/PLG160-g-m PEG2K（CDDP-NPs） accelerated with the increase of the acidity of the environment. In vitro test demonstrated that CDDP-NPs could inhibit the proliferation of MNNG/Hos osteosarcoma cells with IC50（72 h） of 12.2 μg·mL^-1. In vivo test for MNNG/Hos osteosarcoma tumor bearing mice exhibited that CDDP-NPs had comparable or slightly higher efficacy but significantly lower side effects in comparison with free CDDP. The coadministration of i RGD could further enhance the anticancer efficacy of CDDP-NPs against MNNG/Hos osteosarcoma without bringing obvious side effects. Therefore, CDDP-NPs using alone or with iRGD have great potential for the treatment of osteosarcoma.Herein, cisplatin-loaded poly（L-glutamic acid）-g-methoxy poly（ethylene glycol） nanoparticles were evaluated as a potential chemotherapeutic agent against osteosarcoma by using alone or with an i RGD（internalizing RGD, CRGDKDPDC）. The release rate of platinum from the cisplatin-loaded nanoparticles CDDP/PLG160-g-m PEG2K（CDDP-NPs） accelerated with the increase of the acidity of the environment. In vitro test demonstrated that CDDP-NPs could inhibit the proliferation of MNNG/Hos osteosarcoma cells with IC50（72 h） of 12.2 μg·mL^-1. In vivo test for MNNG/Hos osteosarcoma tumor bearing mice exhibited that CDDP-NPs had comparable or slightly higher efficacy but significantly lower side effects in comparison with free CDDP. The coadministration of i RGD could further enhance the anticancer efficacy of CDDP-NPs against MNNG/Hos osteosarcoma without bringing obvious side effects. Therefore, CDDP-NPs using alone or with iRGD have great potential for the treatment of osteosarcoma.

关 键 词：Nanoparticles Biodegradable polymers Cancer chemotherapy Polymeric drug carrier 

分 类 号：O631.3[理学—高分子化学] TQ460.1[理学—化学]