RP-HPLC测定白屈菜药材中白屈菜碱的含量  

Determination of Chelidonine in Chelidonium majus Using Reversed-phase High Performance Liquid Chromatography

作　　者：曲中原[1] 王嘉琪[2] 邹晓祺 刘影[2] 石鑫[1] 邹翔[3] 

机构地区：[1]哈尔滨商业大学药学院 [2]国家教育部抗肿瘤天然药物工程研究中心 [3]哈尔滨商业大学药物所博士后科研工作站,哈尔滨150076

出　　处：《天然产物研究与开发》2015年第3期462-465,共4页Natural Product Research and Development

基　　金：国家自然科学基金青年基金(81102858);中国博士后科学基金(2013M531060);黑龙江省博士后科研基金(LBH-Z11103);黑龙江省骨干教师项目(1154G35);黑龙江省自然科学基金(D200817)

摘　　要：选用超声波、热回流和索氏3种提取方法,甲醇、丙酮和氯仿3种提取溶剂提取白屈菜中的白屈菜碱,考察最佳提取方法和提取溶剂,采用高效液相色谱法测定白屈菜碱含量。采用Diamonsil C18反相色谱柱为分析柱,以乙腈-1%三乙胺溶液（20∶80）（三乙胺用磷酸调节p H至3.0）为流动相,流速为1 m L/min,检测波长为290nm。获得最佳提取方法为索氏提取法,最佳提取溶剂为丙酮,测得白屈菜中白屈菜碱的含量为0.118%。白屈菜碱在0.01~0.10 mg/m L范围内呈良好线性关系,r=0.9999（n=6）,通过方法学考察,回收率为97.48%±1.46%,RSD为1.50%。实验结果表明丙酮索式提取法能较全面的提取白屈菜中的白屈菜碱,利用高效液相色谱法含量测定,简便快速,准确可靠,可用于白屈菜药材及其制剂的质量控制。In order to select the best extraction method and extraction solvent,the extraction methods including ultrasonic,hot reflux and soxhlet and the extraction solvents including methanol,acetone and chloroform were used to extract chelidonine from Chelidonium majus. The content of chelidonine was determined by HPLC. Diamonsil C18 reverse phase column was used as chromatographic analysis column. The mobile phase was acetonitrile-1% triethylamine（ 20 ∶ 80）（ adjusted p H to 3 using phosphoric acid） and the UV detection wavelength was 290 nm. The best extraction method was determined to be soxhlet extraction and the best extraction solvent was acetone. The content of chelidonine in C. majus was 0. 118%. The linear range of chelidonine was 0. 01-0. 10 mg / m L（ r = 0. 9999,n = 6）. The average recovery was97. 48% ± 1. 46%,RSD was 1. 50%. Experimental results showed that acetone soxhlet extraction method can more comprehensively extract chelidonine in C. majus. The ddeveloped HPLC method was simple,rapid and accurate. It can be used for quality control of C. majus and its preparations.

关 键 词：白屈菜 白屈菜碱 提取方法 提取溶剂 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]