四种绿绒蒿属植物中总黄酮含量测定  

Determination of Total Flavonoids in Four Meconopsis Species from Qinghai-Tibet Plateau

作　　者：舍莉萍[1,2] 孙菁[1] 周玉碧[1] 孙胜男[1,2] 赵庆帅[1,2] 叶润蓉[1] 卢学峰[1] 彭敏[1] 

机构地区：[1]中国科学院西北高原生物研究所青海省青藏高原特色生物资源研究重点实验室,西宁810008 [2]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2015年第3期466-469,共4页Natural Product Research and Development

基　　金：中国科学院"西部之光"人才培养计划(2010);中国科学院知识创新工程重要方向(KSCX2-EW-J-26);国家科技支撑计划(2010BAK68B02)

摘　　要：本文采用比色法测定了4种绿绒蒿属植物中总黄酮的含量,该方法线性关系良好(r2=0.9990),平均回收率为101.56%,精密度和重复性试验RSD分别为2.94%和1.22%,表明本试验的重复性较好。结果显示总黄酮含量以五脉绿绒蒿中最高,全缘和红花绿绒蒿次之,总状绿绒蒿中最低,4种药材总黄酮含量的变化与其用途差异基本吻合,与藏医用药品次习惯较为相符。本实验结果可以为绿绒蒿属药用植物资源的深入研究和开发利用提供一定的理论依据。In this paper,the contents of total flavonoids in four Meconopsis species collected from the Qinghai-Tibet plateau were determined by ultraviolet-visible spectrophotometer with colorimetric method. This method showed good linearity（ r2= 0. 9990）. The average recovery of the method was 101. 56%; the accuracy and repeatability of the method were confirmed by the precision and repetition tests with RSD = 2. 94% and 1. 34%,respectively. The results showed that the total flavonoids content in Meconopsis quintuplinervia was the highest,which was a top-grade medicinal herb in accord with the traditional usage habit of Tibet. In addition,the results showed that there was a remarkable difference between Meconopsis quintuplinervia and the others which was analyzed by SPSS 20. 0 with ANOVA method. The results presented in this study,will provide a theoretical basis for the further studies of the medicinal herbs of Meconopsis species.

关 键 词：总黄酮 比色法 五脉绿绒蒿 全缘绿绒蒿 红花绿绒蒿 总状绿绒蒿 

分 类 号：R927.2[医药卫生—药学]