锁阳配伍维生素C对大鼠运动性肾脏缺血再灌注的保护作用  

Protective Effect of Cynomorium songarium Rupr Combined with Vitamin C on Exercised-induced Renal Ischemia-reperfusion Injury of Rats

作　　者：崔笑梅[1] 曹建民[2] 周海涛[3] 

机构地区：[1]兰州理工大学,兰州730050 [2]北京体育大学,北京100084 [3]北京联合大学生物化学工程学院,北京100023

出　　处：《天然产物研究与开发》2015年第3期506-511,共6页Natural Product Research and Development

摘　　要：本文对锁阳配伍维生素C对大鼠运动性肾缺血再灌注损伤的保护作用及机制进行了研究。实验中对大鼠以4.5 g/kg锁阳灌胃,100 mg/kg的维生素C腹腔注射56 d,并进行力竭游泳训练及血尿素氮等生化指标测定。结果显示,8周的过度训练导致大鼠运动性肾缺血"再灌注",肾组织组织病理学发生明显改变,肾功能受到严重损坏。血尿素氮和血清肌酐各组较对照组明显上升,与OM组相比,COM组、VCOM组、CVCOM组明显下降,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,COM组、CVCOM组明显下降,分别为P<0.05、P<0.01。SOD活性各组较对照组明显降低,与OM组相比,COM组、VCOM组、CVCOM组明显上升,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,COM组、CVCOM组明显上升,分别为P<0.05、P<0.01。MDA含量各组较对照组明显升高,与OM组相比,COM组、VCOM组、CVCOM组明显下降,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,COM组、CVCOM组明显降低,分别为P<0.05、P<0.01。从而说明锁阳和维生素C均可以增强SOD活性,清除氧自由基,减轻脂质过氧化作用,对运动性缺血再灌注肾脏具有保护作用。锁阳作用优于维生素C。二者配伍使用效果更好。This paper studied the protective effect and mechanism of Cynomorium songarium Rupr（ CSR） combined with vitamin C on exercised-induced renal ischemia-reperfusion injury of rats. The experimental rats were gavaged with 4. 5g /kg of CSR and intraperitoneal injected with 100 mg / kg of vitamin C for 56 days（ 8 weeks）,which was followed by exhaustive swimming training. After that,the blood urea nitrogen and other biochemical indexes of the rats were determined. The results showed that 8 weeks of overtraining led to exercised-induced renal ischemia-reperfusion injury in rats. Moreover,the rats＇ kidney histopathology was changed significantly,and renal function was severely damaged. Compared with the control group,each group of blood urea nitrogen and serum creatinine increased significantly. Compared with OM group,COM group,VCOM group and CVCOM group decreased significantly（ P〈0. 05,P〈0. 05,P〈0. 01）;compared with VCOM group,COM group and CVCOM group decreased significantly（ P〈0. 05,P〈0. 01）. Compared with the control group,the activity of SOD in each group was significantly decreased. Compared with OM group,COM group,VCOM group and CVCOM group increased significantly（ P〈0. 05,P〈0. 05,P〈0. 01）; compared with VCOM group,COM group and CVCOM group increased significantly（ P〈0. 05,P〈0. 01）. The content of MDA in each group was significantly higher than that of the control group,compared with OM group,COM group,VCOM group and CVCOM group decreased significantly（ P〈0. 05,P〈0. 05,P〈0. 01）; compared with VCOM group,COM group and CVCOM group significantly decreased（ P〈0. 05,P〈0. 01）. The experimental results showed that CSR and vitamin C can enhance the activity of SOD,scavenge oxygen free radicals,and reduce lipid peroxidation. It had a protective effect on exercise-induced ischemia-reperfusion kidneys. The effect of CSR was better than that of vitamin C. The combined usage of CSR and vitamin C provided better effect.

关 键 词：锁阳 维生素C 运动性缺血再灌注 

分 类 号：R285.5[医药卫生—中药学]