盆架树中1个新单萜吲哚生物碱  

A new monoterpenoid indole alkaloid from Winchia calophylla

作　　者：曾婷[1] 李小年[2] 李丽梅[1] 

机构地区：[1]成都医学院科研实验中心,四川成都610500 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201

出　　处：《中草药》2015年第5期629-632,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21172024);教育部科学技术研究重点项目(212151);四川省科技计划项目青年基金(2014JQ0049)

摘　　要：目的研究盆架树Winchia calophylla树皮的生物碱成分。方法采用硅胶和Sephadex LH-20柱色谱等方法进行分离和纯化,通过MS、NMR等波谱数据以及X-射线单晶衍射分析进行结构鉴定,采用MTT法测试化合物对7种人体肿瘤细胞(肺癌A549,乳腺癌MCF-7,前列腺癌PC-3,神经胶质瘤U87MG,多发性骨髓瘤U266、MM1.S、MM1.R)增殖的抑制作用。结果从盆架树树皮乙醇提取物的总生物碱部分分离得到1个新单萜吲哚生物碱,命名为盆架树碱B(1)。结论化合物1为新化合物,该化合物对7种人体肿瘤细胞株无增殖抑制作用。Objective To investigate the alkaloid constituents from the trunk barks of Winchia calophylla.Methods The constituents were isolated and purified by column chromatography over silica gel and Sephadex LH-20 columns.The structure of the isolated compound was elucidated by MS and NMR spectra,and then confirmed by a single-crystal X-ray crystallographic analysis.The isolated compound was also evaluated for its in vitro cytotoxicity against the proliferation of seven kinds of human cancer cell lines（lung cancer：A549,breast cancer：MCF-7,prostate cancer：PC-3,glioma：U87 MG,multiple myeloma：U266,MM1.S,and MM1.R）.Results A new monoterpenoid indole alkaloid,identified as calophyline B（1）,was isolated from the ethanol extract of the trunk barks of W.calophylla.Conclusion Compound 1 is a new compound,which shows no cytotoxicity against the proliferation of the above human tumor cells.

关 键 词：夹竹桃科 盆架树 单萜吲哚生物碱 X-射线单晶衍射分析 盆架树碱B 

分 类 号：R284.1[医药卫生—中药学]