嘎日迪-13对局灶性脑缺血大鼠NSE、TNF-α、IL-6表达的影响  

Effect of Gridi-13 on expression of NSE,TNF-α,and IL-6 after focal cerebral ischemia in rats

作　　者：田彩云[1] 张浩楠[1] 何静波[1] 武海军[1] 覃建民[1] 徐继辉[1] 杨玉梅[1] 

机构地区：[1]包头医学院心血管研究室,内蒙古包头014040

出　　处：《中草药》2015年第5期716-720,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81160560)

摘　　要：目的探讨血清中神经元特异性烯醇化酶(NSE)、炎性因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)在大鼠脑缺血损伤后的作用及嘎日迪-13对其影响。方法取雄性SD大鼠360只,将大鼠随机分为脑缺血模型组、假手术组及嘎日迪-13大、中、小剂量(0.24、0.12、0.06 g/kg)组,每组又随机分为缺血1、6、12、24、72、120 h的6个时相组,采用改良Zea Longa线栓法制备大鼠大脑中动脉栓塞(MCAO)模型,ELISA法检测血清中NSE、TNF-α、IL-6水平。结果NSE、TNF-α、IL-6在假手术组表达少;而在模型组脑缺血损伤1 h后开始上升,NSE于12 h达高峰,TNF-α、IL-6在24 h达高峰,之后逐渐下降(P<0.01);嘎日迪-13各剂量治疗组能显著降低神经功能评分,NSE、TNF-α、IL-6表达量明显受到抑制(P<0.01、0.05)。结论嘎日迪-13对大鼠脑缺血损伤具有明显的保护作用,提示嘎日迪-13可能是通过减轻脑缺血损伤后神经元的损伤,调节炎症因子相互之间的作用,减轻脑组织损伤,从而发挥对脑缺血损伤的治疗作用。Objective To investigate the roles of neuron-specific enolase（NSE）,tumor necrosis factor-α（TNF-α）,and interleukins-6（IL-6） in serum on focal cerebral ischemic injury of rats as well as the effect of Gridi-13 on them.Methods A total of 360 male SD rats were selected.They were randomly divided into model group,Sham-operated group,Gridi-13 large dose group,Gridi-13 middle dose group,and Gridi-13 small dose group.Each group was randomly divided into 1,6,12,24,72,and 120 h time phase groups.The middle cerebral artery occlusion（MCAO） model was established by modified Zea Longa thread-occlusion method and the contents of NSE,TNF-α,and IL-6 in serum were determined by ELISA method.Results The expression of NSE,TNF-α,and IL-6 was very weak in Sham-operated group while rising after the cerebral ischemic injury for 1 h compared with the model group.The peak values of NSE,TNF-α,and IL-6 appeared at 12,24,and 24 h,respectively and then going down gradually（P 0.01）.Gridi-13 in each dose group could predominantly reduce the nervous function score and the expression levels of NSE,TNF-α,and IL-6 were remarkably inhibited（P 0.01 and P 0.05）.Conclusion Garidi-13 has the significant protection on the cerebral ischemia injury in rats,which may be related with alleviating neuronal damage,regulating inflammatory factors,and relieving the injury of brain tissue,so as to play a role of therapeutic action in cerebral ischemic injury of rats.

关 键 词：嘎日迪-13 脑缺血 神经元特异性烯醇化酶 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]