刺参种子后熟调控技术研究  

Study on the control technology for after-ripening of the Oplopanax elatus Nakai seeds

作　　者：冯颖[1] 闫中雪 顾地周[1] 沈红梅[1] 徐鹏佳 袁浩[1] 张力凡[1] 

机构地区：[1]通化师范学院生命科学学院,吉林通化134002

出　　处：《浙江大学学报（理学版）》2015年第2期175-180,共6页Journal of Zhejiang University（Science Edition）

基　　金：吉林省科技发展计划项目(201500920)

摘　　要：以刺参种子为供试材料,通过多因子交叉重复实验对影响刺参种子后熟的主要因素进行了筛选,并确定了其影响水平.采用2.50mg·L^-1 GA3和2.60-2.80mg·L^-12,4-D混合溶液浸泡48h,将种子与河砂（1∶3）混合,变温处理140-147d,白天温度为（22±2）℃,处理14h,夜间温度为（8±2）℃,处理10h.结果表明,心形胚发生率为98.6%,子叶胚发生率为95.7%,播种后发芽率达96.0%.获得了刺参种子的后熟条件,可应用于刺参的育苗.The seeds of Oplopanax elatus were used as test materials,and the main factors at different levels for after-ripening seeds of Oplopanax elatus were investigated through repeated factorial experiments with cross.The results show that the seed of Oplopanax elatus are soaked in the mixed solutions with 2.50mg·L^-1 GA3 and 2.60-2.80mg·L^-12,4-D for 48 h,and mixed with the river sand at the ratio of 1∶3in the poikilothermic treatment for140-147d.The extent of temperature changes are（22±2）℃ for 14 hby day and（8±2）℃ for 10 hat night.The incidence rate of the heart-shape embryo is 98.6%;the incidence rate of the embryo with cotyledons is 95.7%;the germination percentage is 96.0%.In this study,the after-ripening conditions of the Oplopanax elatus seeds are obtained,which are applicable to the seedlings of Oplopanax elatus.

关 键 词：刺参 集成后熟 调控技术 形态发育 

分 类 号：S567[农业科学—中草药栽培]