Molecular Characteristics and Drug Susceptibility of Mycobacterium tuberculosis Isolates from Patients Co-infected with Human Immunodeficiency Virus in Beijing,China  

作　　者：LIU Jie WANG Hui Zhu LIAN Lu Lu YU Yan Hua ZHAO Xiu Qin GUO Cai Ping LIU Hai Can LIU Shu Mei ZHAO Hui ZENG Zhao Ying ZHAO Xiu Ying WAN Kang Lin 

机构地区：[1]National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control [2]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases [3]Chaoyang Center for Disease Control and Prevention [4]Beijing Ditan Hospital,affiliated to Capital Medical University [5]Beijing You'an Hospital,affiliated to Capital Medical University [6]Beijing Tsinghua Changgung Hospital [7]Medical Center of Tsinghua University

出　　处：《Biomedical and Environmental Sciences》2015年第3期222-226,共5页生物医学与环境科学（英文版）

基　　金：funded by(2013ZX10003006-002-001 and 2011ZX10004-001)National Key Program of Mega Infectious Disease;National Natural Science Foundation(30973388);Capital Medical Development Scientific Research Fund(2009-1057)

摘　　要：70 clinical Mycobacterium tuberculosis strains isolated from AIDS patients in two HIV/AIDS referral hospitals in Beijing were used in this study.M.tuberculosis and non-tuberculosis mycobacterium（NTM）were identified by using multi-locus PCR.M.tuberculosis was genotyped by using 15-locus MIRU-VNTR technique and spoligotyping afterwards.Meanwhile,the drug susceptibilities of the strains to the four first-line anti TB drugs（rifampin,isoniazid,streptomycin, and ethambutol） and the four second-line anti-TB drugs （capreomycin, kanamycin, ofloxacin, and ethionanide） were tested with proportional method. In this study, M. tuberculosis and NTM strains isolated from AIDS patients with TB-like symptoms were identified and genotyping analysis indicated that Beijing genotype was the predominant genotype. In addition, the prevalence of drug-resistant TB, especially the prevalence of XDR-TB, was higher than that in TB patients without HIV infection.70 clinical Mycobacterium tuberculosis strains isolated from AIDS patients in two HIV/AIDS referral hospitals in Beijing were used in this study.M.tuberculosis and non-tuberculosis mycobacterium（NTM）were identified by using multi-locus PCR.M.tuberculosis was genotyped by using 15-locus MIRU-VNTR technique and spoligotyping afterwards.Meanwhile,the drug susceptibilities of the strains to the four first-line anti TB drugs（rifampin,isoniazid,streptomycin, and ethambutol） and the four second-line anti-TB drugs （capreomycin, kanamycin, ofloxacin, and ethionanide） were tested with proportional method. In this study, M. tuberculosis and NTM strains isolated from AIDS patients with TB-like symptoms were identified and genotyping analysis indicated that Beijing genotype was the predominant genotype. In addition, the prevalence of drug-resistant TB, especially the prevalence of XDR-TB, was higher than that in TB patients without HIV infection.

关 键 词：tuberculosis Mycobacterium locus infected VNTR inappropriate isolates genotype ofloxacin belonged 

分 类 号：R512.91[医药卫生—内科学]