华蟾素对多发性骨髓瘤RPMI 8226细胞株增殖、凋亡的影响及机制研究  

Proliferation-Inhibiting and Apoptosis-Inducing Influence of Cinobufacini on Human Myeloma RPMI 8226 Cells and Its Mechanism of Action

作　　者：李宏良[1] 田华琴[1] 曾荣香[1] 梁贵文[1] 黄小青[1] 陈学彰[1] 王斌[1] 杨耀林[1] 王艳杰[1] 黄泳立[1] 

机构地区：[1]佛山市中医院肿瘤血液科,广东佛山528000

出　　处：《辽宁中医杂志》2015年第3期456-458,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：广东省中医药局科研课题(20111061)

摘　　要：目的:探讨华蟾素对多发性骨髓瘤RPMI 8226细胞株增殖、凋亡的影响及作用机制。方法:分别采用四氮唑蓝(MTT)比色法、流式细胞仪检测华蟾素对多发性骨髓瘤RPMI 8226细胞增殖、凋亡的影响。用ELISA检测细胞上清液中白细胞介素-6(IL-6)及血管内皮生长因子(VEGF)水平。结果:1不同浓度华蟾素对RPMI 8226细胞的增殖均有抑制作用,随药物浓度的增加及作用时间的延长,药物的作用效果增强;2不同浓度华蟾素(0.0625、0.125、0.25、0.5μg/m L)作用于RPMI 8226细胞72h,细胞凋亡率分别为(14.92±1.59)%、(19.17±2.10)%、(19.43±2.63)%和(27.47±3.39)%,与对照组(2.98±0.67)%比较,差异有统计学意义(P<0.01)。0.5μg/m L浓度华蟾素凋亡率高于其于各组(P<0.01);3与对照组比较,不同浓度华蟾素作用于RPMI 8226细胞72h,细胞上清中IL-6及VEGF水平下降,其中高浓度华蟾素组(0.25及0.5μg/m L)的变化有显著性差异(P<0.01),但低浓度组(0.0625及0.125μg/m L)变化无统计学意义(P>0.05)。结论:华蟾素在体外具有抗多发性骨髓瘤的作用,并呈时间和剂量的依赖性,与其直接细胞毒作用及诱导凋亡有关,降低瘤细胞分泌IL-6及VEGF的水平可能是其作用机制之一。Objective： To investigate the proliferation-inhibiting and apoptosis-inducing effects of cinobufacini on human myeloma RPMI 8226 cells and its mechanisms. Methods： Cell proliferation was measured by methylthiazolyl tetrazolium（MTT）assay. The rates of cell apoptosis were evaluated by flow cytometry（FCM）. The IL-6 and VEGF concentrations of cell supernatants were tested by ELISA. Results： 1Cinobufacini showed significant effect of inhibiting proliferation on RPMI8226 cells at various concentrations from 0. 0625 to 0. 5μg / m L in a time and dose-dependent manner（P〈0. 01） and it also showed significant effects on cell apoptosis rate（P〈0. 01） when cultured with cinobufacini at the concentrations for 72 h in a dose-dependent manner. The rate of cell apoptosis in 0. 5μg / m L cinobufacini group was significantly higher than that of the other groups. Compared with that in the control group,the IL-6 and VEGF concentrations were down-regulated after acted by 0. 25 and 0. 5 μg /m L cinobufacini（P〈0. 01） but the levels of IL-6 and VEGF in 0. 0625 and 0. 125 μg / m L cinobufacini groups had no significant change（P〉0. 05）. Conclusion： Cinobufacini can inhibit the proliferation of RPMI 8226 cells in a time and dose-dependent manner,which may be relevant to cytotoxic effect and promoting apoptosis. Its mechanisms may be correlated with the levels IL-6 and VEGF secretion down-regulating.

关 键 词：华蟾素 多发性骨髓瘤 增殖 凋亡 白细胞介素-6 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]