电针曲池、上巨虚对UC模型大鼠ChAT与a7nAChR等表达的影响  

Effect of Electro-acupuncture at Quchi(LI11) and Shangjuxu(ST37) on Levels of ChAT and α7nAChR

作　　者：杨璐佳 张泓[1] 张雨辰[2] 邓石峰 王珍珍[1] 粟艳梅[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410007 [2]湖南中医药大学第二附属医院,湖南长沙410007

出　　处：《辽宁中医杂志》2015年第3期635-637,I0003,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科技基金项目(81173327/H2718)

摘　　要：目的:观察电针溃疡性结肠炎(UC)模型大鼠大肠经经合穴"曲池"、下合穴"上巨虚"后对肿瘤坏死因子-α(TNF-α)、乙酰胆碱转移酶(ChAT)、烟碱型乙酰胆碱受体α7(α7nAChR)表达的影响并对其相关作用机制进行分析,初步探讨"合治内府"中"合"穴的相对特异性。方法:以TNBS(2-4-6三硝基苯磺酸)/乙醇溶液灌肠诱导建立SD大鼠UC模型,曲池组与上巨虚组造模成功后分别电针曲池穴和上巨虚穴,连续治疗10 d,末次治疗24 h后处死所有组大鼠,肉眼观察大鼠结肠溃疡评分,提取血清、结肠组织并制备标本,检测其ChAT,α7nAChR,TNF-α的表达。结果:1与空白组相比:模型组溃疡评分、TNF-α表达明显升高(P<0.01),ChAT,α7nAChR表达明显降低(P<0.01)。2与模型组相比:曲池组TNF-α表达明显降低(P<0.01)、α7nAChR表达明显升高(P<0.01),结肠溃疡评分降低和ChAT表达升高均有趋势但无统计学意义(P>0.05);上巨虚组溃疡评分、TNF-α的表达明显降低(P<0.01),ChAT、α7nAChR的表达明显升高(P<0.01)。3与曲池组相比:上巨虚组溃疡评分、TNF-α表达明显降低(P<0.01),ChAT、α7nAChR表达水平明显升高(P<0.05或0.01)。结论:1电针曲池、上巨虚均能降低UC模型大鼠结肠溃疡评分和TNF-α的表达,升高ChAT、α7nAChR的表达,说明电针对溃疡性结肠炎的治疗作用可能是通过调节TNF-α、ChAT、α7nAChR等来实现的;2比较而言:电针上巨虚的治疗作用优于曲池,说明"上巨虚"存在相对特异性;3本研究结果部分证实:"合治内府"中的"合"主要应指下合穴,其具体内涵仍需进一步研究。Objective： To observe the expression levels of tumor necrosis factor（TNF-α）,choline acetyltransferase（ChAT）and alpha 7 nicotinic acetylcholine receptor r（α7n AChR） in the ulcerative colitis（UC） rats and study the related mechanism to further understand the meaning of He points in treating viscera diseases by He points after electro-acupuncturing at Quchi（LI11） and Shangjuxu（ST37）. Methods： The UC rat models were given trinitrobenzene sulphoneacid（TNBS） /ethanol enema.And then Quchi（LI11） group and Shangjuxu（ST37） group respectively received electro-acupuncture at Quchi（LI11） and Shangjuxu（ST37） for 10 consecutive days. 24 h after the last treatment,we dissected all rats and collected serum specimen and colon tissue to observe ulcer score and the expression levels of ChAT,α7n AChR and TNF-α. Results： 1Compared with the control group,ulcer score and TNF-α significantly increased（P〈0. 01） and ChAT and α7n AChR significantly decreased（P〈0.01） in model group. 2Compared with the model group,Quchi（LI11） group made obvious increase in a7 n AChR（P〈0. 01） and decrease in TNF-α（P〈0. 01）. And the ulcer score had a downward trend and ChAT level an upward trend（P〈0. 05） without significant difference. Compared with the model group,the expression of TNF-α and ulcer score significantly reduced（P〈0. 01） and ChAT and α7n AChR increased significantly（P〈0. 01） in Shangjuxu（ST37） group. 3Compared with Quchi（LI11）group,ulcer score and TNF-α decreased significantly（P〈0. 01） and the ChAT and α7n AChR level of Shangjuxu（ST37）group increased significantly（P〈0. 05 or P〈0. 01）. Conclusion： 1 Both of the large intestinal meridian＇ s lower He point Shangjuxu（ST37） and upper He point Quchi（LI11） can improve the UC through decreasing the ulcer score and TNF-α and increasing the levels of ChAT and α7n AChR. 2The treatment effect of Shangjuxu（ST37） on the UC is better than Quchi（LI11）.It shows

关 键 词：电针 UC模型大鼠 合穴 合治内府 胆碱能抗炎通路 

分 类 号：R245.97[医药卫生—针灸推拿学]