桂芍知母汤对类风湿关节炎模型鼠滑膜组织血管内皮生长因子的影响  

Influence of Guishao Zhimu decoction on vascular endothelial growth factor in sliding membrane tissue of rheumatoid arthritis model rats

作　　者：孙乾[1] 王金春[1] 孙锋[1] 

机构地区：[1]沈阳市第五人民医院风湿免疫科,沈阳110023

出　　处：《实用药物与临床》2015年第3期253-255,共3页Practical Pharmacy and Clinical Remedies

摘　　要：目的观察桂芍知母汤对胶原诱导类风湿关节炎(CIA)模型鼠血管内皮生长因子(VEGF)的影响,探讨其作用靶点。方法将40只健康雌性大鼠随机分为正常组、模型组、来氟米特组、桂芍知母汤组,除正常组之外,其余各组使用Ⅱ型胶原诱导大鼠造成CIA模型。桂芍知母汤组、来氟米特组,造模后开始给药,共计30 d。按评分法评价关节炎指数,ELISA法检测VEGF表达。结果给药组较模型组关节发病数目减少,关节肿胀程度减轻,皮肤红肿不明显,关节变形程度减轻。给药组与模型组在关节炎指数方面比较有明显差异(P<0.05)。给药组CIA大鼠踝关节滑膜组织升高的VEGF水平显著降低(P<0.01)。结论桂芍知母汤组与模型组比较,关节炎指数明显降低,大鼠踝关节滑膜组织VEGF水平明显下降。Objective To observe the influence of Guishao Zhimu decoction on vascular endothelial growth factor（ VEGF） in rheumatoid arthritis（ CIA） model of mice induced by collagen,and discuss the acting targets. Methods 40 healthy female rats were randomly divided into normal group,model group,fluorine mitt group and Guishao Zhimu decoction group. All groups were induced by using Ⅱ type collagen to form the CIA model in rats except the normal group. The arthritis index was evaluated by using score method,vascular endothelial growth factor（ VEGF） expression was evaluated by using ELISA method in Guishao Zhimu decoction group and fluorine mitt group after 30 d of treatment. Results Compared with model group,the numbers of joint disease reduced,the degree of joint swelling relieved,skin irritation disappeared,the degree of joint deformation alleviated in the medication group. There was significant difference in arthritis index between the medication groups and model group（P〈0. 05）. The increased levels of VEGF in ankle synovial tissue of rheumatoid arthritis significantly reduced in medication group（P〈0. 01）.Conclusion Compared with model group,the arthritis index and ankle synovial tissue VEGF levels of rats in Guishao Zhimu decoction group significantly decreased.

关 键 词：桂芍知母汤 类风湿关节炎 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]