调节性T细胞在小鼠实验性自身免疫性肌炎模型中的表达及意义  

Role of regulatory T cells in a mouse model of experimental autoimmune myositis

作　　者：石强[1] 田成林[1] 刘洁晓[2] 蒲传强[2] 

机构地区：[1] 解放军总医院海南分院神经内科,海南省三亚市572000 [2] 解放军总医院神经内科

出　　处：《中国医药》2015年第4期592-595,共4页China Medicine

基　　金：北京市科技计划（Z111107058811108）

摘　　要：目的 探讨调节性T细胞（Treg）与实验性自身免疫性肌炎（EAM）的关系.方法 将20只BALC/c小鼠完全随机分为EAM组和刘照组,各10只.EAM组皮下注射0.25 ml弗氏完全佐剂＋0.25 ml20 μg/μl骨骼肌匀浆,对照组皮下注射0.25 ml弗氏完全佐剂＋0.25 ml磷酸盐缓冲液,观察2组小鼠肌肉组织形态学变化和CD45表达.流式细胞仪检测2组小鼠外周血及脾脏单个核细胞中CD4＋CD25＋ Foxp3＋ Treg所占CD4＋T细胞的比例.结果 EAM组小鼠肌肉组织HE染色可见大量炎性细胞浸润,伴有肌纤维萎缩、坏死、吞噬,CD45表达阳性.对照组未见炎性细胞浸润.EAM小鼠外周血单个核细胞中CD4＋CD25＋Foxp3Treg所占CD4 T细胞的比例低于对照组小鼠[（8.0±4.O）％比（15.5±6.9）％],脾脏单个核细胞中CD4＋CD25 Foxp3＋ Treg所占CD4 T细胞的比例低于对照组小鼠[（4.7±2.2）％比（8.6±4.0）％],差异均有统计学意义（均P＜0.05）.结论 Treg细胞群在维持机体免疫平衡中起重要作用,为特发性炎性肌病免疫机制的研究及治疗提供了新思路.Objective To observe the proportion of CD4 CD2~ Foxp3 regular T cells （Tregs） for CD4 T ceils in peripheral blood and spleen mononuelear ceils in a mouse model of experimental autoimmune myositis （EAM） ; to explore the relation between Tregs and autoimmune myositis, Methods Totally 20 BALC/c mice were randomly divided into EAM group administrated with Freund＇s complete adjuvant （ CFA, 0.25 ml） ＋ skeletal muscle homogenates （20 μg/μl, 0.25 ml）, and control group that had CFA （0.25 m]） ＋ phosphate buffer solution （0.25 ml）. The morphological changes of muscle tissue and expression of CD45 were observed, and the proportions of CD4＋ CD2＋ Foxp3 Tregs for CD4 T ceils in peripheral blood and spleen mononuelear cells were measured by flow eytometry. Results In EAM group, HE staining showed abundant inflammatory cell infiltration with expression positive of CD45 , accompanied by atrophy, necrosis, phagocytosis of muscle fiber; no inflammatory cell infiltration was observed in control group. The proportions of CD4 CD2＋ Foxpf Tregs for CD4 T cells in peripheral blood and spleen mononuelear cells in EAM group were both significantly lower than those in control group[ （8.0± 4.0）% vs（15.5±6.9）%, （4.7±2.2）% vs （8.6±4.0）%,P〈0.05]. Conclusion Treg cells play an important role in maintaining immune balance, which provides a new idea for research and treatment of the idiopathic inflammatory myopathies.

关 键 词：实验性自身免疫性肌炎 CD4+CD25+Foxp3+调节性T细胞 流式细胞仪 

分 类 号：R593[医药卫生—内科学]