酶标分析仪波长标准物质的研制  

Preparation of Interference Filter Reference Materials for ELISA Analytical Instruments

作　　者：杨欣欣[1] 张彬[1] 荀其宁[1] 拓锐[1] 胡国星[1] 潘忠泉[1] 

机构地区：[1]中国兵器工业集团第五三研究所,济南250031

出　　处：《化学分析计量》2015年第2期6-9,共4页Chemical Analysis And Meterage

摘　　要：以硫化锌为高折射材料和冰晶石为低反射材料,利用真空镀膜技术、光学极值法检测膜厚技术镀制了窄带全介质干涉滤光片,K9玻璃为薄膜保护层,采用切割、粗磨、精磨、抛光、镀膜等光学工艺制得酶标分析仪波长标准物质,经均匀性、稳定性考核合格后,由紫外可见近红外分光光度计标准装置对标准物质定值。研制的酶标分析仪波长标准物质定制结果扩展不确定度为0.7 nm(k=2),使用方便,量值准确、稳定,技术指标满足酶标分析仪波长示值误差及重复性检定/校准工作的需要。The interference filter reference materials were made by vacuum coating technology and optical film thickness extremum method which plating zinc sulfide as high refraction materials and cryolite as low reflective materials of narrow–band interference filter, and K9 glass was used as film protective layer. After some optical technology such as cutting, coarse grinding,fine grinding,polishing and plating, the uniformity, stability of interference filter reference materials were examinated by the ultraviolet–visible–near infrared spectrophotometer standard device. According to the results, the deterministic uncertainty of the development reference material was 0.7 nm（k=2）. Therefore, the developed interference filter reference materials can meet the verification/calibration requirement for wavelength error and repeatability of ELISA analytical instruments with convenience and stable value.

关 键 词：酶标分析仪 波长 标准物质 均匀性 稳定性 

分 类 号：O651[理学—分析化学]