紫尿酸显色分光光度法测定盐酸氯丙嗪片剂含量  

Content Determination of Chlorpromazine Hydrochloride Cablet by Spectrophotometry with Thivioluric Acid as Chromogenic Reagent

作　　者：万能勇 黄选忠[2] 

机构地区：[1]湖北兴山县南阳镇卫生院,湖北兴山443713 [2]湖北省兴山县疾病预防控制中心,湖北兴山443700

出　　处：《化学分析计量》2015年第2期75-78,共4页Chemical Analysis And Meterage

摘　　要：建立了以紫尿酸为显色剂测定盐酸氯丙嗪的光度法。在弱酸性条件下，Fe（Ⅲ）被盐酸氯丙嗪定量还原成Fe（Ⅱ），Fe（Ⅱ）在碱性条件下与紫尿酸反应形成不稳定的蓝色配阴离子，该配阴离子与盐酸氯丙嗪形成稳定的蓝色离子缔合物，该离子缔合物的最大吸收波长位于635 nm，其表观摩尔吸光系数ε=2.62×104 L／（mol·cm），盐酸氯丙嗪质量浓度在0.5～10.0 mg／L范围内与体系的吸光度呈良好的线性关系，线性相关系数为0.9992。测定结果的相对标准偏差为1.02%～2.65%（n=5），加标回收率为91.0%～95.0%。该法灵敏度较高、选择性及重现性良好，可用于片剂中盐酸氯丙嗪含量的测定。A new method for the determination of trace chlorpromazine hydrochloride by the spectrophotometry with violuric acid as chromogenic agent was established. In the medium of weak acid, Fe（Ⅲ） was chlorpromazine hydrochloride quantitative reduction of Fe（Ⅱ）, Fe（Ⅱ） reacted with violuric acid to form a blue complex anion in alkaline conditions, and the complex anion and chlorpromazine hydrochloride formed stable blue ionic associate, the maximum absorption wavelength was 635 nm, the apparent molar absorptivity coefficient was 2.62×104 L/（mol·cm）, and the absorbance was linear with the chlorpromazine hydrochloride content in the range of 0.5–10.0 mg/L with the correlation coefficient of 0.999 2. The relative standard deviation of the detection results was 1.02%–2.65% （n=5）, and the standard addition recovery rate was 91.0%–95.0%. This methodhas the advantages of high sensitivity, good selectivity and reproducibility, and it can be used for the determination of chlorpromazine hydrochloride in tablets.

关 键 词：紫尿酸 分光光度法 盐酸氯丙嗪 

分 类 号：O657.3[理学—分析化学]