正交试验结合HPLC法筛选太白洋参提取工艺  

Extraction Techniques for Pedicularis Decora Franch Screening by Orthogonal Test and HPLC

作　　者：颜永刚[1] 吴玲[1] 马妮[1] 李博[1] 王玉[1] 薛泉[1] 

机构地区：[1]陕西中医学院药学院,陕西咸阳712046

出　　处：《陕西中医学院学报》2015年第2期74-77,共4页Journal of Shaanxi College of Traditional Chinese Medicine

基　　金：陕西省科技厅(2011JQ4011);国家级大学生创新创业训练计划项目:太白洋参抗老年性痴呆活性物质基础指纹图谱研究;陕西省药品食品管理局项目:太白洋参药材质量标准研究

摘　　要：目的筛选太白洋参中化学成分提取的最佳工艺条件。方法采用HPLC法,以太白洋参中桃叶珊瑚苷相对含量为评判指标,通过正交试验设计,考察乙醇浓度,提取时间、提取次数、温度等因素对太白洋参中桃叶珊瑚苷化学成分的影响,确定最佳提取条件。结果用水提取:温度80℃;提取次数3次;提取时间90 min的效果最好。用乙醇提取:乙醇浓度60%;提取次数2次;提取时间120 min的效果最好。结论经综合分析提取最佳工艺为乙醇醇浓度60%,溶媒用量为6倍,回流时间120 min,提取次数2次。该研究方法综合中药化学整体特征,为提取工艺评价方法提供借鉴。太白洋参中桃叶珊瑚苷提取工艺的优化,为太白洋参中桃叶珊瑚苷进一步研究提供参考。Objective： To screen the optimum technological conditions for extracting the chemical compositions in pedicularis decora franch. Methods： The comparative contents of aucubin in screened pedicularis decora franch as the evaluation indexes by HPLC,the influence of the factors like ethanol concentrations,extraction time,extraction frequencies and temperatures over the chemical composition of aucubin in pedicularis decora franch were observed by orthogonal test to determine the optimum conditions. Results： The optimum conditions of extraction by water： 80℃,3 times and 90minutes; the optimum conditions of extraction by ethanol： 60% concentration,twice and 120 minutes. Conclusion：Through comprehensive analysis,the optimum extraction conditions are that the concentration of ethanol is 60%,solvent volume is 6 times,the reflux hour is 120 minutes and the extraction frequency is twice. The research method provides a lesson for evaluation of the extraction technologies,combined the overall features of Chinese medical chemistry. The optimum techniques of extracting aucubin from pedicularis decora franch provide references for its further research.

关 键 词：太白洋参 正交试验 HPLC 桃叶珊瑚苷 提取工艺 

分 类 号：R284.2[医药卫生—中药学]