子宫内膜异位症患者腹腔液中蛋白质质谱的变化  

Changes of peritoneal fluid protein mass spectrometry of patients with endometriosis

作　　者：贾艳艳[1] 史惠蓉[1] 任芳[1] 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052

出　　处：《郑州大学学报（医学版）》2015年第2期248-251,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:了解子宫内膜异位症(简称内异症)患者腹腔液中蛋白质质谱变化。方法:取内异症患者30例、非内异症患者30例的腹腔液,采用SELDI-TOF-MS技术进行蛋白质质谱检测并结合生物信息学方法进行分析。结果:内异症组与非内异症组腹腔液蛋白质质谱间7个蛋白质差异有统计学意义(P均<0.05)。M/Z为9 022.578和6 516.219的2个蛋白质被自动作为分类变量建立决策树分类模型,其敏感性为93.33%(28/30),特异性为83.33%(25/30)。其中M/Z为9 022.578的蛋白质在内异症患者腹腔液中的表达降低,而M/Z为6 516.219的蛋白质在内异症患者腹腔液中的表达升高。结论:从内异症患者腹腔液中筛查出7种差异蛋白质,对进一步研究内异症的病因及发病机制有重要意义。Aim：To analyse the protein mass spectrometry in the peritoneal fluid of endometriosis patients .Methods：Samples were taken from the peritoneal fluid of patients （ 30 cases of endometriosis , and 30 cases of non-endometriosis ） . Protein mass spectrometry were analysed using SELDI-TOF-MS.Results：A total of 7 protein peaks were tested with statis-tic significance in the peritoneal fluid between the two groups （P〈0.05）.Two proteins, with M/Z of 9 022.578 and 6 516 .219 respectively , were selected for a diagnostic model of endometriosis .The sensitivity and specificity of the model were 93.33%（28/30） and 83.33%（25/30） respectively.The protein with M/Z of 9 022.578 was down-regulated, and that with M/Z of 6 516 .219 was up-regulated in endometriosis patients .Conclusion：The screened 7 protein have impor-tant value in further study on etiology and pathogenesis of endometriosis .

关 键 词：子宫内膜异位症 蛋白质质谱 腹腔液 

分 类 号：R711.71[医药卫生—妇产科学]