湘酿1号刺葡萄离体快繁技术试验  

Rapid propagation technology of Xiangniang-1(Vitis davidii Foёx) in vitro

作　　者：莫银屏 徐丰[1] 石雪晖[1] 杨国顺[1] 刘昆玉[1] 金燕[1] 钟晓红[1] 

机构地区：[1]湖南农业大学园艺园林学院/湖南省葡萄工程技术研究中心,长沙410128

出　　处：《中外葡萄与葡萄酒》2015年第2期26-28,共3页Sino-Overseas Grapevine & Wine

基　　金：现代农业产业技术体系建设专项资金(CARS-30);湖南省科技成果转化与产业化项目(2014CK1005);湖南省研究生科研创新项目(CX2013B291);湖南省教育厅一般项目(13C387)

摘　　要：以刺葡萄品种湘酿1号的单芽茎段为材料,通过采用不同消毒方法和生根处理,以建立高效的离体快繁体系。结果表明:单芽茎段用1 mol/L HCl处理30 s后,再用0.1%升汞消毒8 min,接种在加入了20 mg/L四环素培养基中,能取得最优消毒效果;最优外植体诱导培养基为MS+6-BA 1.00 mg/L+IBA0.25 mg/L。与其他葡萄品种相比,"湘酿1号"生根能力弱。The single bud green cuttings from Xiangniang-1（Vitis davidii Foёx） was used as materials to establish the rapid propagation system in vitro. The results showed that the green cuttings with single bud could obtain the best disinfection by 1 mol/L HCl for 30 s, and 0.1% HgCl2 for 8 min, finally them were inoculated in solid medium with 20 mg/L tetracycline. The optimum inducing medium on these explants was MS＋6-BA 1.0 mg/L＋IBA 0.25 mg/L. Compared with the other varieties, Xiangniang-1＆#39;s rooting ability was weak.

关 键 词：湘酿1号 刺葡萄 单芽茎段 离体培养 

分 类 号：S663.1[农业科学—果树学]