Chemical Analysis of Xueshuantong Lyophilized Powder by LC-MS Profiling  

作　　者：Ming Chen Wen-zhi Yang Wan-ying Wu De-an Guo 

机构地区：[1]Guangxi Wuzhou Pharmaceutical (Group) Co.,Ltd. [2]Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences

出　　处：《Chinese Herbal Medicines》2015年第1期54-61,共8页中草药（英文版）

基　　金：Twelfth Five-Year National Science&Technology Support Program(2012BA129B06)

摘　　要：Objective To elucidate the chemical components of Xueshuantong （XST） Lyophilized Powder and primarily disclose the chemical difference between XST and Panax notoginseng roots. Methods Liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-ESI-MSn） was used to profile the saponins in XST and P. notoginseng. Structural elucidation was based on spectral analyses of negative and positive ESI-MS3 data, and the comparison of the retention behaviors. Results The optimized LC-MS profiling approach enabled resolution of major saponins. The negative mode ESI-MS3 fragmentation gave diagnostic information on the nature （neutral loss 162 Da for GIc, 146 Da for Rha, and 132 Da for a pentose） and sequence （priority： terminal 〉 inner） of sugars and sapogenins （m/z 475 for protopanaxatriol; re^z459 for protopanaxadiol）, while the intact glycosyl portion could be characterized by characteristic Zoα, Cnnα＋, and Cnβ＋ （n = 2 or 3） obtained in the positive mode. Ultimately, a total of 30 saponins were characterized from XST. Compared with the roots of P. notoginseng, three malonyl-ginsenosides, ginsenoside Rd, and gyponoside XVII （or its isomer） were almost undetectable, and showed potential significance for their differentiation. Conclusion The established LC-MS profiling approach is powerful for the chemical analysis of P. notoginseng and its preparations such as XST.Objective To elucidate the chemical components of Xueshuantong （XST） Lyophilized Powder and primarily disclose the chemical difference between XST and Panax notoginseng roots. Methods Liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-ESI-MSn） was used to profile the saponins in XST and P. notoginseng. Structural elucidation was based on spectral analyses of negative and positive ESI-MS3 data, and the comparison of the retention behaviors. Results The optimized LC-MS profiling approach enabled resolution of major saponins. The negative mode ESI-MS3 fragmentation gave diagnostic information on the nature （neutral loss 162 Da for GIc, 146 Da for Rha, and 132 Da for a pentose） and sequence （priority： terminal 〉 inner） of sugars and sapogenins （m/z 475 for protopanaxatriol; re^z459 for protopanaxadiol）, while the intact glycosyl portion could be characterized by characteristic Zoα, Cnnα＋, and Cnβ＋ （n = 2 or 3） obtained in the positive mode. Ultimately, a total of 30 saponins were characterized from XST. Compared with the roots of P. notoginseng, three malonyl-ginsenosides, ginsenoside Rd, and gyponoside XVII （or its isomer） were almost undetectable, and showed potential significance for their differentiation. Conclusion The established LC-MS profiling approach is powerful for the chemical analysis of P. notoginseng and its preparations such as XST.

关 键 词：FINGERPRINT LC-MS Panax notoginseng  saponins XUESHUANTONG 

分 类 号：R286.0[医药卫生—中药学] O657.63[医药卫生—中医学]