吉林省2000-2012年急性弛缓性麻痹病例中脊髓灰质炎病毒的鉴定  

Identification of Acute Flaccid Paralysis Patients' Polioviruses in Jilin Province,2000-2012

作　　者：范明[1] 刘桂艳[1] 周剑惠[1] 王爽[1] 魏雷雷[1] 林琳[1] 郭立春[2,3] 单元春[1] 曹凤瑞[1] 严冬梅[2] 无 

机构地区：[1]吉林省疾病预防控制中心,吉林省卫生厅病毒重点实验室,长春130062 [2]中国疾病预防控制中心,北京100050 [3]天津市西青区疾病预防控制中心,天津300380 [4]中国疾病预防控制中心病毒病预防控制所,卫生部医学病毒学与病毒病重点实验室,世界卫生组织西太平洋区脊髓灰质炎参比实验室,北京102206

出　　处：《中国疫苗和免疫》2015年第1期24-28,共5页Chinese Journal of Vaccines and Immunization

基　　金：吉林省卫生厅急性弛缓性麻痹病例中肠道病毒研究项目(编号:2008Z086)

摘　　要：目的分析吉林省2000-2012年从急性弛缓性麻痹（Acute Flaccid Paralysis,AFP）病例粪便标本中分离的脊髓灰质炎（脊灰）病毒（Poliovirus,PV）的血清分型以及型内鉴别结果。方法原始粪便标本来自吉林省2000-2012年﹤15岁儿童AFP病例,使用转人PV受体的小鼠肺细胞系和人横纹肌肉瘤细胞进行病毒分离,阳性标本进行中和试验鉴定血清型,中和试验鉴定为阳性的PV株送中国疾病预防控制中心病毒病预防控制所国家脊灰实验室进行型内鉴别,型内鉴别方法采用聚合酶链反应-限制性酶切片段长度多态性分析和酶联免疫吸附试验或VP_1编码区核苷酸序列分析法。结果吉林省2000-2012年共采集804例AFP病例粪便标本,通过细胞培养的方法共有25例分离到PV,均为疫苗相关（Vaccine-associated）PV（VAPV）,其中2008年1例AFP病例分离到Ⅱ型和Ⅲ型VAPV,其中的Ⅲ型PV为VP1编码区Ⅲ型和Ⅱ型疫苗重组PV。结论吉林省2000-2012年未发现脊灰野病毒或疫苗衍生脊灰病毒引起的AFP病例,为吉林省维持无脊灰提供了实验室依据。Objective To analyze serotype and intratype of acute flaccid paralysis （AFP） patients＇ polio- virus strains from Jilin province during 2000 - 2012. Methods From 2000 to 2012, stool specimens were collected from AFP patients less than 15 years old. Virus isolation was performed by inoculating a mouse cell line expressing the gene for the human cellular receptor for poliovirus （L20B） and a human rhabdomyosarcoma （RD） cell line. Positive samples were used to conduct serotype identification with the neutralization test. Samples positive in the neutralization test were sent to the National Polio Laboratory for intratypic differentiation （ITD） using polymerase chain reaction-restriction fragment length polymor- phism （PCR-RFLP） and enzyme linked immunosorbent assay （ELISA）, or sequence analysis of the VP1 coding region. Results In total, 804 stool specimens were collected, and 25 strains were obtained by cell culture. All strains were vaccine associated poliovirus （VAPV）, except one, a sample from 2008 that was confirmed to be a type 2 and 3 vaccine hybrid strain. Furthermore, the type 3 was a type3/type2 intertypic vaccine-related strain recombinant with the crossover site within the VP1 capsid coding region. Conclusion No wild polioviruses or vaccine derived polioviruses （VDPV） were detected through AFPsurveillance. Polio-free status was supported by laboratory-based evidence.

关 键 词：急性弛缓性麻痹 脊髓灰质炎病毒 血清分型 型内鉴别 

分 类 号：R373.33[医药卫生—病原生物学]