鬼针草滴眼液治疗兔围绝经期干眼症的实验研究  

Experimental study on efficiency of Spanishneedles Herb eye drops in treating perimenopausal xerophthalmia in rabbits

作　　者：邵毅[1] 余瑶[2] 余静[3] 裴重刚[1] 高桂平[1] 涂萍[2] 

机构地区：[1]南昌大学第一附属医院眼科,江西南昌330006 [2]南方医科大学附属南昌市第三医院内分泌科,江西南昌330009 [3]浙江中医药大学杭州市中医药针灸科,浙江杭州310053

出　　处：《中国中药杂志》2015年第6期1151-1155,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81160118,81400372);全国临床医药专项基金项目(L2012052);江西省科技平台建设项目(2013116);江西省青年科学基金项目(20114BAB215036);江西省科技支撑计划项目(20111BBG70026-2);江西省卫计委科研项目(20155154);江西省教育厅青年科技计划项目(GJJ14170);江西省远航工程(2014022)

摘　　要：目的:研究鬼针草滴眼液对围绝经期性激素水平下降导致的干眼症的治疗作用。方法:选择新西兰白兔36只(36眼,均为右眼),制作去卵巢模型2月后随机分为3组(各12例):实验组(A组)使用鬼针草滴眼液滴眼,对照组(B组)使用PBS滴眼,模型组(C组)不使用任何眼液滴眼。分别于治疗前和治疗后1周、2周、1月、2月各时间点检测基础泪液分泌试验(Schirmer I test,SIT)、角膜荧光素染色(fluorescent,FL)、泪液总蛋白含量、淀粉酶活性、乳铁蛋白、溶菌酶含量及角膜共焦显微镜扫描。结果:治疗前A,B 2组SIT,FL,泪液总蛋白量,乳铁蛋白,溶菌酶及淀粉酶活性相比,差异无统计学意义;治疗2月后,B组SIT,FL,泪液总蛋白量,乳铁蛋白,溶菌酶及淀粉酶活性较治疗前均有不同程度改变,差异有统计学意义(P<0.05);A组SIT,FL,泪液总蛋白量,乳铁蛋白,溶菌酶及淀粉酶活性均有不同程度改变,差异有统计学意义(P<0.05);A,B 2组各时间点SIT,FL,泪液总蛋白量,乳铁蛋白,溶菌酶及淀粉酶活性相比,差异均有统计学意义(P<0.05)。治疗2月后,A组上皮基底细胞和炎症细胞密度分别为(4 122±416),(339±131)个/mm2,而B组上皮基底细胞和炎症细胞密度分别为(3 343±424),(49±17)个/mm2,2组比较,差异均有统计学意义(P<0.05)。结论:鬼针草滴眼液能有效治疗围绝经期性激素水平下降导致的兔干眼症,具有很好的临床应用前景。Objective： To investigate the efficiency of Spanishneedles Herb eye drops in treating perimenopausal xerophthalmia in rabbits. Method： Totally 36 rabbits（ 36 right eyes） were ovariectomized,and 2 months later divided into three groups： the experimental group（ group A,n = 12） given Spanishneedles Herb eye drops,the control group（ group B,n = 12） given PBS and the model group（ group C,n = 12） given no drug. The Schirmer I test（ SIT）,fluorescent（ FL）,total tear protein,diastase activity,lactoferrin and lysozyme contents and confocal scanning microscopy were performed at before the treatment and at 1 w,2 w,1 mo,2 mo after the treatment. Result： Before the treatment,There was no significant difference in SIT,FL,total tear protein,lysozyme,lactoferrin and amylase activity between two groups. Two months later after the treatment,both the group B and the group A showed differences degrees of changes in SIT,FL,total tear protein,lysozyme,lactoferrin and amylase activity compared with that before the treatment,with statistical differences（ P〈0. 05）; At each time point,both groups revealed statistical differences in SIT,FL,total tear protein,lysozyme,lactoferrin and amylase activity（ P〈0. 05）. Two months later after the treatment,densities of basal epithelial cells and inflammatory cells in the group A were（ 4 122 ± 416） cells / mm2and（ 339 ± 131） cells / mm2,while that in the group B were（ 3 343 ± 424）cells / mm2and（ 49 ± 17） cells / mm2,with statistical differences between them（ P〈0. 05）. Conclusion： Spanishneedles Herb eye drops could effectively treat perimenopausal xerophthalmia in rabbit caused by sex hormones decline.

关 键 词：鬼针草滴眼液 干眼 治疗 雌激素 

分 类 号：R285.5[医药卫生—中药学]