PEG渗透胁迫对苦豆子幼苗生理的影响  

Effects of PEG osmotic stress on seedling physiology of Sophora alopecuroides

作　　者：段启荣 刘丹[1] 王雪[1] 刘晶[1] 李亚斌[1] 刘萍[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《农业科学研究》2015年第1期20-24,共5页Journal of Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(131074909);宁夏自然科学基金项目(NZ14033)

摘　　要：以PEG-6000模拟干旱条件,测定苦豆子幼苗叶片质膜相对透性、丙二醛(MDA)含量、脯氨酸(Pro)含量以及抗过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性.结果表明随干旱胁迫程度的加剧,苦豆子叶片质膜相对透性逐渐增高,叶片中MDA含量先降低后升高最后又有所下降,长时间重度胁迫促使脯氨酸含量显著增加;POD、CAT和SOD 3种保护酶先后交替发挥作用,胁迫初期以POD和CAT为主,胁迫中期以POD与SOD为主,胁迫后期以CAT和SOD为主.相关分析表明,Pro含量与质膜相对透性、SOD活性、POD活性呈极显著或显著正相关,CAT活性与POD活性、MDA含量极显著负相关.苦豆子幼苗对干旱胁迫有较强的适应能力.The physiological activity and drought-resistant capability of Sophora.alopecuroides seedlings were studied under the simulated condition of drought by PEG-6000. The Results showed that with increasing stress intension, the relative permeability of plasma membrane rose gradually, malonaldehyde（MDA） content in leaves of S.alopecuroides seedlings showed a decrease-increase-decrease trend. The proline is observed to accumulate in severe stress for a long time.Three kinds of protective enzyme, namely peroxidase（POD）, catalase（CAT） and superoxide dismutase（SOD）, function alternatively in the whole PEG stress; initially, POD and CAT dominate the protective process, followed by POD and SOD at the mid stage of stress, and CAT and SOD in the later stage. The correlation analysis among the aforementioned indexes showed significant positive correlation between proline content and relative permeability of plasma membrane, and the activities of SOD and POD.Significantly negative correlation is found between activities of CAT and activities of POD and MDA content. S.alopecuroides seedlings could adapt to the solution drought stress conditions.

关 键 词：苦豆子 PEG胁迫 幼苗生理 相关性分析 

分 类 号：Q945.78[生物学—植物学] R282.2[医药卫生—中药学]