丽格海棠的组织培养  

The tissue culture of Rieger begonia

作　　者：金雯[1] 彭佳佳[1] 秦扬[1] 刘春[2] 张黎[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]中国农科院蔬菜花卉研究所,北京100081

出　　处：《农业科学研究》2015年第1期65-68,共4页Journal of Agricultural Sciences

摘　　要：本试验以丽格海棠"巴克斯"和"桑德尼"2个品种的幼嫩叶片、带叶柄叶片为外植体进行组织培养,分别研究不同激素质量浓度以及不同培养条件对丽格海棠愈伤组织诱导、不定芽分化、增殖培养、诱导生根的影响.结果表明:MS+6-BA 0.05 mg/L+NAA 0.2 mg/L适合于愈伤组织的诱导,2个品种的诱导率分别为100%和91.7%.MS+6-BA 0.1 mg/L+NAA 0.05 mg/L适合于不定芽增殖培养基.分化率为81.2%,1/2 MS+IBA0.2 mg/L适合于诱导生根,生根率达100%.同时,研究出增殖阶段外植体最适生长环境,为丽格海棠的组织培养快速繁殖提供了重要依据.In this experiment,Young Leaves and leaves with petioles of Begonia rieger ＇Barkos＇ and ＇Sang Deni＇ as explants for microprapagation. callus inducing,bud differentiation and proliferation of adventitious bud, rootage inducing were studied under culture media with different concentration of hormones and culture conditions. The result showed： MS＋6-BA0.05mg/L＋NAA0.2mg/L is suitable for inducing callus, induction rate of two varieties are 100% and 91.7%.MS＋6-BA0.1mg/L＋NAA 0.05mg/L is suitable for bud proliferation, the rate of bud regeneration is 81%.The optimum rooting medium for：1/2 MS ＋IBA0.2 mg/L, the rooting rate achieves 100%.And suitable conditions for stage of proliferation of adventitious bud are found out.The study will provide important basis for the rapid propagation of Begonia rieger by tissue culture.

关 键 词：丽格海棠 外源激素 组织培养 

分 类 号：Q813.11[生物学—生物工程]