活血通脉颗粒对颈动脉粥样硬化家兔ICAM-1、IL-6表达的影响  

Effect of the Huoxue Tongmai Granule on ICAM-1 and IL-6 of Arotid Atherosclerosis Rabbit

作　　者：谢健[1] 童晓云[2] 沈超[2] 李晓[1] 石安华[2] 陈文玲[2] 刘涛[1] 和春芳[1] 王雅莉[1] 赵淳[1] 

机构地区：[1]云南省中医医院云南中医学院第一附属医院,云南昆明650021 [2]云南中医学院,云南昆明650500

出　　处：《中国中医急症》2015年第3期396-398,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：云南省自然科学基金资助项目(2011FZ264);国家中医药管理局"名老中医药专家工作室"建设项目

摘　　要：目的探讨活血通脉颗粒干预兔颈动脉粥样硬化(CAS)形成的作用机制。方法 75只新西兰白兔,随机抽取10只为正常组,其余65只采用高脂饲料喂养建立CAS模型。造模成功后将其随机分为模型组、自然消退组、活血通脉颗粒低、中、高剂量组、辛伐他汀组6组,每组7只。分别于实验第11周、第16周取颈动脉组织行HE染色以观察病理变化;ELISA法检测细胞间黏附分子-1(ICAM-1)、白介素-6(IL-6)表达水平;荧光定量PCR检测ICAM-1 m RNA表达。结果与自然消退组比较,活血通脉颗粒中、高剂量组和辛伐他汀组ICAM-1、IL-6水平明显下降(P<0.05),与活血通脉颗粒低剂量组比较,活血通脉颗粒高剂量组、辛伐他汀组明显下降(P<0.05);与自然消退组比较,各治疗组ICAM-1 m RNA表达明显下降(P<0.05)。结论活血通脉颗粒可能通过抑制ICAM-1、IL-6表达水平从而干预家兔动脉粥样硬化的形成。Objective： To invigorate the mechanism of Huoxue Tongmai Granule intervention the rabbit with carotid atherosclerosis（CAS）.Methods： In the 75 New Zealand white rabbit,randomly selected 10 for the normal group,the remaining 65 rabbits were established by the high-fat feed carotid atherosclerosis model.After 11 weeks,the models were randomly divided into the model group,the natural healing group,the Huoxue Tongmai Granule low,medium and high dose group,simvastatin group with 7 rabbits in each group.11 weeks experiment respectively,and week 16 carotid line organization HE staining was used to observe the carotid artery tissue pathological changes.Meanwhile,ELISA method for detection of ICAM- 1 and IL-6 expression were dectected by the ELISA method.ICAM-1 m RNA expression was observed by the fluorescence quantitative PCR detection.Results： Compared with the natural healing group,ICAM-1 and IL-6 expression was decreased obviously in the Huoxue Tongmai Granule middle and high dose group（P〈0.05）.Compared with Huoxue Tongmai Granule lowdose group,the high-dose group and the simvastatin group were significantly decreased（P〈0.05）.Compared with the natural healing group,the m RNA expression of ICAM- 1 was significantly decreased in the treatment group（P〈0.05）.Conclusion： Huoxue Tongmai Granule could inhibit ICAM-1and IL-6 expression level for the intervention of the formation of atherosclerosis.

关 键 词：活血通脉颗粒 颈动脉粥样硬化 ICAM-1 IL-6 兔 

分 类 号：R285.5[医药卫生—中药学]