HIV-1抗体阳性者WB带型及首次CD4^+T淋巴细胞检测分析  

Analysis of bands patterns in Western Blot and first CD4^+T lymphocytes count of HIV-1 antibody positive patients

作　　者：魏爱旺[1] 陆祥彬[1] 张俊岭[1] 马智龙[1] 

机构地区：[1]江苏省泰州市疾病预防控制中心,泰州225300

出　　处：《江苏预防医学》2015年第2期18-19,共2页Jiangsu Journal of Preventive Medicine

摘　　要：目的通过对新发现HIV-1抗体阳性者WB带型、首次CD4+T淋巴细胞计数结果的分析,了解其免疫状况及疾病进展情况。方法运用免疫印迹试验、流式细胞仪检测HIV-1阳性者静脉血中的抗体、CD4+T淋巴细胞。结果 162例HIV-1抗体阳性者WB带型以全条带和次全带为主,CD4+T淋巴细胞计数<350/μL的占49。38%。HIV-1抗体全条带与p55条带出现率在不同免疫状况组差异有统计学意义(P<0.05)。结论 HIV-1抗体阳性者的WB带型、CD4+T淋巴细胞计数可帮助判断机体免疫状态、确定疾病分期、监测疾病进展。Objective To understand the immune status and disease progression by analyzing WB bands patterns and first CD4＋T lymphocytes count of the newly identified HIV-1 antibody positive patients.Methods Antibodies and CD4＋T lymphocytes of HIV-1 antibody positive patients were analyzed by western blot（WB）and flow cytometry,respectively.Results The WB bands patterns of 162HIV-1 antibody positive patients were mainly total bands or subtotal bands;of which 49.38% were with 〈350/μL CD4＋T lymphocytes.The occurrence of HIV-1antibody total bands pattern and p55 band were significant different among groups of different immune status（P〈0.05）.Conclusion The WB bands patterns and CD4＋T lymphocyte counts can help to judge immune status,determine disease stages and monitor disease progression.

关 键 词：HIV-1抗体 免疫印迹试验 WB带型 CD4+T淋巴细胞 免疫状况 

分 类 号：R512.91[医药卫生—内科学]