CdTe量子点对人乳腺癌细胞MCF-7/ADM的化学增敏作用及其机制研究  

Mechanisticanalysis of CdTe quantum dots-enhanced chemosensitization of MCF-7/ADM cells

作　　者：钟文英[1] 李晓明[2] 韩玉萍[1] 陈楠[2] 黄庆[2] 赵云[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《四川大学学报（自然科学版）》2015年第2期397-402,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31100716;U1332119)

摘　　要：利用纳米材料CdTe量子点作为增敏剂,四甲基偶氮唑蓝(MTT)法检测细胞活力的实验表明CdTe量子点增强了阿霉素对于MCF-7/ADM的毒性,而对非耐药的MCF-7无增敏作用.然后利用免疫印迹法和免疫荧光染色法检测发现CdTe量子点提高了MCF-7/ADM细胞的自噬水平,且这种自噬水平的上调与化学增敏效应是相关的,以上结果提示CdTe量子点通过诱导MCF-7/ADM细胞发生自噬,增强耐药性细胞对DOX毒性的敏感性.CdTe quantum dots（QDs）were utilized as a sensitizer,Cell viability via MTT assay indicated that CdTe QDs enhanced the sensitivity to DOX for MCF-7/ADM cells but not for non-resistant MCF-7cells.Further studies using immunoblotting and immunofluorescence microscopy revealed that CdTe QDs up-regulated autophagy in MCF-7/ADM cells,and increased level of autophagy are related to their ability of chemosensitization.These results suggest that autophagy is part of the machanism for CdTe QDs-induced chemosensitization.

关 键 词：乳腺癌 耐药性 阿霉素 CDTE量子点 增敏 自噬 

分 类 号：G25[文化科学—图书馆学]