拟南芥AtRAC在盐胁迫应答中的功能研究  

Functionalanalysis of arabidopsis AtRAC in response to saltstress

作　　者：翟先芝 杨虹伟[1] 杨皓[1] 刘志斌[1] 王健美[1] 杨毅[1] 李旭锋[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2015年第2期416-422,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金项目(31171586;31300996;30971816)

摘　　要：本实验所研究的拟南芥AtRAC含有两个RING结构域和三个蛋白激酶C保守区域1结构域即C1结构域.通过体外泛素化证明了AtRAC具有E3连接酶活性,亚细胞定位实验结果表明AtRAC定位在细胞核中.组织特异性分析表明AtRAC在根中的表达量很高,但是在茎、叶和花中的表达量较低,而且AtRAC的表达受到NaCl胁迫的诱导.进一步对atrac突变体的分析表明,在NaCl处理下,突变体atrac的萌发率降低,侧根数目也降低.初步研究表明拟南芥AtRAC可能是一个与盐胁迫应答相关的基因.Arabidopsis AtRAC contains two RING structure domains and three protein kinase C conservative area 1structures namely C1 structure domain.In vitro self-ubiquitination assay demonstrated that this RING domain protein possessed an E3 ligase activity.In this paper,subcellular localization,gene expression profiles and tissue specificity of it were carried out.The result showed that AtRAC was localized to nucleus.The analysis of expressional pattern revealed that AtRAC was highly expressed in root,less expressed in leaves,flower and stems.NaCl stress on seed germination and lateral root formation were also analyzed.And the result revealed that knockout of AtRAC in Arabidopsis showed low germination rate and less lateral root.These studies demonstrated that AtRAC was related to salt stress.Thus indicating that the RING Finger Protein AtRAC may play an important physiological role in salt stress process.

关 键 词：E3连接酶 亚细胞定位 表达谱分析 组织特异性 盐胁迫 

分 类 号：Q945[生物学—植物学]