番茄SlWRKY1转录因子在植物生物和非生物胁迫中的调控  

The regulation of SlWRKY1 transcription factor in biotic and abiotic stress in Solanum lycopersicum

作　　者：张凝[1] 高永峰[1] 孙晓春[1] 刘永胜[1,2] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室、水力学与山区河流开发保护国家重点实验室,成都610064 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《四川大学学报（自然科学版）》2015年第2期435-440,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家973计划项目(2011CB100401);国家自然科学基金(31171179);教育部博士点优先资助基金项目(20110181130009);国家杰出青年科学基金(30825030)

摘　　要：克隆番茄SlWRKY1基因,构建植物过量表达载体pBI121-35S∷SlWRKY1并通过农杆菌介导的遗传转化法转化番茄,成功获得3株过量表达的转基因植株.研究发现:转基因植株对丁香假单胞菌番茄变种(Pst DC3000)的感染表现出敏感性,表明SlWRKY1基因可以减弱由Pst DC3000引起的植物防御反应,在相关信号通路中起到负调控作用.同时SlWRKY1转基因植株对盐胁迫表现出抗逆性,在胁迫条件下转基因植物中积累了大量的脯氨酸.推测SlWRKY1基因可能参与番茄脯氨酸代谢调控.SlWRKY1gene was Cloned from Genome of tomato.In addition,an over-expression vector composing of aimed gene fragment and pBI121 with CaMV35 S promoter,named pBI121-35S∷SlWRKY1was constructed.This vector was introduced into tomato cv.Ailsa Craig by Agrobacterium-mediated transformation,and then acquired 3independent transgenic plants of over-expressing.We found that all transgenic plants were sensitive to infection of P.syringae pv.tomato DC3000（Pst DC3000）,suggested that SlWRKY1 genes can weaken plant defense responses and be as a negative regulatory factor in signaling pathways.Meanwhile SlWRKY1 gene also exhibited resistance to salt stress and caused massive accumulation of proline in transgenic plants under stress conditions.It was speculated that SlWRKY1 gene may participate in proline metabolism regulation mechanism.

关 键 词：番茄 WRKY转录因子 SlWRKY1 干旱胁迫 PST DC3000 盐胁迫 

分 类 号：Q75[生物学—分子生物学] S641.2[农业科学—蔬菜学]