超高效液相色谱-串联四极杆飞行时间质谱法快速鉴别生晒参中的皂苷类成分  

Rapid analysis of ginsenosides in dried fresh Ginseng by ultra-perfor-mance liquid chromatography with quadrupole time-of-flight mass spectrometry

作　　者：张晓旭[1,2] 王洪平[2] 杨阳[3] 杜茂波[2] 毛森[4] 陈畅[2] 刘玉欣[1] 李韶菁[2] 

机构地区：[1]河北大学药学院,河北保定071002 [2]中国中医科学院中药研究所,北京100700 [3]中国中医科学院,北京100700 [4]北京中医药大学东直门医院,北京100700

出　　处：《中国医药导报》2015年第9期130-136,共7页China Medical Herald

基　　金：国家科技重大专项"重大新药创制"项目(编号2014ZX09304307001;2012ZX09103201-055);国家自然科学基金资助项目(编号81303261;81274133);中国中医科学院中药研究所基本科研业务费自主选题项目(编号ZZ2014060;ZZ2014005)

摘　　要：目的采用超高效液相色谱与串联四极杆飞行时间质谱联用技术,快速全面鉴别生晒参中人参皂苷类成分。方法色谱分离采用Agilent Zorbax RRHD Eclipse Plus C18色谱柱;流动相为水(含0.1%甲酸,A)和乙腈(含0.1%甲酸,B),梯度洗脱。质谱定性采用高分辨飞行时间质谱,电喷雾双喷离子源(Dual AJS ESI),负离子模式。结果11 min内对生晒参中的95个皂苷类成分进行了鉴别。结论本研究为目前生晒参的质量控制提供了可行的依据。Objective To identify the saponins components from dried fresh Ginseng by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry rapidly and comprehensively. Methods Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase was consisted of 0.1% formic acid water（A） and 0.1% formic acid acetonitrile（B） with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight spectrometer mass which equipped with a Dual AJS ESI source operating in negative mode. Results A total of 95 ginsenosides in dried fresh Ginseng were identified only in 11 minutes. Conclusion This study provides a feasible basis for the quality control of the dried fresh Ginseng.

关 键 词：人参 人参皂苷 超高效液相色谱 四极杆飞行时间质谱 

分 类 号：R284.1[医药卫生—中药学]