不完全弗氏佐剂诱导的髓样细胞抑制T细胞活性  

Incomplete Freund's adjuvant-induced myeloid cells inhibit T cell activity

作　　者：刘雨晴[1] 尹宝靓[1] 姚毅波[1] 王子兵[2] 

机构地区：[1]新乡医学院第三附属医院肿瘤科,新乡453003 [2]郑州大学附属肿瘤医院生物治疗科,郑州450008

出　　处：《中国免疫学杂志》2015年第3期344-346,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金(81000914);河南省科技攻关计划重大项目(2011010011)

摘　　要：目的:在肿瘤免疫治疗方法中,肿瘤疫苗前景广阔。在设计肿瘤疫苗时,由于许多肿瘤抗原的免疫原性弱,必须加入一些佐剂才能诱导出机体免疫应答。不完全弗氏佐剂(IFA)是目前肿瘤疫苗研究动物实验中最常用的佐剂之一,已知它能够有效诱导产生高滴度抗体。然而,实验发现,应用含有弗氏佐剂的肿瘤疫苗治疗肿瘤时并未出现期望的结果,原因仍不清楚。方法:BALB/c小鼠接受单次剂量的IFA注射,观察脾脏中CD11b+细胞的比例。使用MTT法检测CD11b+细胞对T细胞增殖的影响和γ干扰素(IFN-γ)分泌能力的影响。结果:IFA可诱导机体产生一群CD11b+细胞,其能抑制T细胞的增殖和IFN-γ分泌。结论:CD11b+细胞可能是导致含有IFA肿瘤疫苗临床效果不佳的原因之一,佐剂的选择会影响肿瘤疫苗的疗效。Objective：As a promising strategy,tumor vaccine is at the forefront of novel approaches to tumor treatment. Since tumor antigens derived from self proteins are often less immunogenic alone, they must be administered with a potent immunostimulatory adjuvant to induce robust T-cell response. IFA was widely used and considered to be one of the most effective adjuvant available for con- sistently producing high titer antibodies to diverse antigens. However, IFA-containing tumor vaccines do not produce positive results. The reasons are unclear. Methods： BALB/c mice were s. c. injected with one single dose of IFA. At indicated days, spleen cells were collected and detected for CDllb cells. MTI method was used to analyze the effect of CDllb~ cells on T cell proliferation. ELISA method was used to determine the influence of CD11 b＋ cell on IFN-~/-secreting ability of T cells. Results：In the spleen of mice treated with IFA,the proportion of CD11 b~ cell was augmented. IFA-induced CD11 b~ cells inhibit the proliferation and tumor antigen-induced IFN-~,-secreting ability of T cells. Conclusion：This finding may help to understand the low therapeutic efficacy of cancer vaccines recently observed in some clinical trials using Freund＇s adjuvant and underscores the necessity of adjuvant selection for active immuno- therapy.

关 键 词：不完全弗式佐剂 肿瘤免疫 免疫治疗 髓样抑制性细胞 

分 类 号：R392[医药卫生—免疫学]