CXCL8及其受体CXCR1、CXCR2在慢性乙肝患者外周血PMNs中的表达  

Expression of CXCL8 and its receptors( CXCR1 and CXCR2 ) in peripheral blood neutrophils of chronic hepatitis B

作　　者：王健[1] 韩忠燕[1] 周娜[1] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,淮南232001

出　　处：《中国免疫学杂志》2015年第3期375-379,383,共6页Chinese Journal of Immunology

基　　金：安徽省自然科学基金(No.1308085MH148;090413138);安徽省教育厅自然科学基金重点项目(No.KJ2010A086)

摘　　要：目的:探讨慢性乙肝患者外周血中性粒细胞(PMNs)上CXCL8及其受体CXCR1、CXCR2的表达。方法:以中性粒细胞分离液分离、纯化PMNs,检测患者血清HBe Ag及PMNs内HBV DNA,入选患者依据检测结果进行分组,SABC免疫细胞化学染色法检测各组患者PMNs内CXCL8及其受体CXCR1、CXCR2的表达。结果:SABC免疫细胞化学染色结果显示,CXCL8主要位于PMNs胞浆中,CXCR1、CXCR2多见于胞浆和胞膜上。其中HBe Ag(+)者CXCL8、CXCR1免疫着色较深,而CXCR2免疫着色较浅;PMNs内HBV DNA(+)者CXCL8、CXCR1免疫着色亦较深,而CXCR2免疫着色亦较浅。患者CXCL8和CXCR1的水平均显著升高,与正常对照相比,差异均有显著性(P<0.05),而CXCR2的表达无统计学意义(P>0.05)。结论:HBV侵染中性粒细胞后可促进CXCL8分泌,使胞膜CXCR1表达进一步增强。CXCL8、CXCR1、CXCR2免疫组化染色程度与患者HBe Ag表达、HBV DNA载量密切相关。高表达CXCR1的中性粒细胞与CXCL8相互作用,趋化吸引更多PMNs至病灶,参与局部炎性损伤和组织修复。Objective ：To study on the levels of CXCL8 and its receptors （ CXCR1 and CXCR2） in peripheral blood neutrophils of the patients with chronic hepatitis B. Methods： The neutrophils were isolated and purified by neutrophil isolation medium, and the loads of HBV-DNA in neutrophils were detected by PCR, and the levels of HBeAg in serum were measured by ELISA. The patients were divided into different groups according to the detective results so that the expressions of CXCL8 and its receptors （ CXCR1, CXCR2） in neutrophils were detected by the methods of streptavidin-biotin complex （ SABC ） immunocytochemistry stain. Results： The data of SABC immunocytochemieal stain showed that the positive color of CXCL8 was mainly located in the cytoplasm of PMNs. However, the most positive color of CXCR1 and CXCR2 was mainly expressed in the cytoplasm and cell membrane. Interestingly, the deeper immune coloring of CXCL8 and CXCR1, and relatively shallow immune coloring of CXCR2 were explored in the group with positive of HBeAg. The similar detective results also had been found in the cases with positive of HBV DNA in neutrophils. Compared with the normal control group, the levels of CXCL8 and CXCR1 in the patients were significantly increased （ P〈O. 05 ） , but the differences were too small to be statistically significant in the level of CXCR2 （P〉0. 05 ）. Conclusion： After neutrophils occult infected by HBV, not only the secretion of CXCL8 can be promoted, but also the expression of CXCR1 will be further increased. The data of immunohistochemical staining have been shown that the color degree of CXCL8 and its receptors （CXCR1, CXCR2 ） are positive correlation to the level ofHBeAg and the loads of HBV DNA. More PMNs can be chemotactic attraction to lesion so as to participate in the local inflammatory injury and tissue repair via the interactive pathway of the high expression of CXCR1 on surface of neutrophils with CXCL8.

关 键 词：慢性乙肝 中性粒细胞(PMNs) CXCL8 CXCR1 CXCR2 免疫细胞化学染色 卵白素-生物素复合物法 

分 类 号：R392.12[医药卫生—免疫学]