多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器  

Surface plasmon resonance sensor coated with poriferous MIPs for microcystin-LR detection

作　　者：何皓[1,2,3] 张涛[1] 姚佳[1] 李传宇[1] 周连群[1] 王毅[4] 李明宇[5] 黎海文[1] 董文飞[1] 

机构地区：[1]中国科学院苏州生物医学工程技术研究所,江苏苏州215163 [2]中国科学院长春光学精密机械与物理研究所,吉林长春130033 [3]中国科学院大学,北京100049 [4]南洋理工大学仿生传感器科学中心,新加坡637553 [5]浙江大学光电信息工程学系,浙江杭州310027

出　　处：《光学精密工程》2015年第3期723-728,共6页Optics and Precision Engineering

基　　金：国家自然科学基金资助项目(No.51202154;No.51205268;No.91123029;No.61340032)

摘　　要：开发了一种多孔分子印迹膜修饰的表面等离子体共振（SPR）传感器,用于快速检测水中微囊藻毒素LR。研究了利用该传感器检测微囊藻毒素LR的方法。首先,通过原位聚合法在SPR传感芯片的裸金表面合成了微囊藻毒素LR的多孔分子印迹膜,制备出可以特异性捕获微囊藻毒素LR的SPR传感芯片。然后,利用Kretschmann棱镜耦合结构,构建了基于Kretschmann结构的波长调制型表面等离子体共振传感器。最后,通过检测不同浓度的微囊藻毒素LR溶液以及干扰物质微囊藻毒素RR溶液,研究了该传感器的测量范围、特异性等参数。结果表明,该传感器对于微囊藻毒素LR的检测灵敏度很高,可实现微囊藻毒素LR的定量检测,动态测量达2.1×10^-9~1×10^-6 mol/L。另外,传感器对于干扰物质微囊藻毒素RR无明显信号响应,表明传感器对于微囊藻毒素LR具有很好的特异性检测能力。A novel surface plasmon resonance（SPR）sensor coated with poriferous molecularly imprinted polymers（MIPs）was developed to detect the trace microcystin-LR in water.The method to detect the trace microcystin-LR in water was explored.Firstly,the in situ self-assembly method was used to synthesize a poriferous thin MIPs film on the gold-plated glass.The SPR chip to specifically capture the microcystin-LR was obtained.Then,based on the typical Kretschmann prism coupling structure,a new type of SPR sensor based on wavelength interrogation was constructed by utilizing the MIPs coated gold-plated glass as the sensing chip.The sensor was used to detect different concentration of microcystin-LR solutions and microcystin-RR solutions and to obtain its measuring ranges and specificity parameters.The result indicates that the sensor is sensitive to microcystin-LR and completes a quantitative determination with a dynamic measure range of 2.1×10^-9—1×10^-6 mol/L.Moreover,the sensor did not have obvious response to microcystin-RR,the analogues of microcystinLR.It means that the sensor has special selectivity to recognize the microcystin-LR.

关 键 词：表面等离子体共振传感器 分子印迹膜 微囊藻毒素LR 水污染检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] X832[自动化与计算机技术—控制科学与工程]