基于海泡石的细胞透性化  

Sepiolite-mediated cell permeabilization

作　　者：王磊[1] 刘玉雪[1] 谭海东[1] 刘武军[1] 赵宗保[1] 

机构地区：[1]中国科学院大连化学物理研究所生物技术部,辽宁大连116023

出　　处：《生物加工过程》2015年第2期57-59,92,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2012CB721103)

摘　　要：利用矿石纳米材料海泡石处理大肠杆菌细胞,建立高效、可逆透性化处理方法。结果表明:海泡石和大肠杆菌BW25113细胞悬液涡旋振荡,细胞通透性增强,90%以上的细胞因渗入博莱霉素致死;而在4℃修复处理过的样品,仅23%的细胞被杀死。发现烟酰胺腺嘌呤二核苷酸(NAD)进出透性化细胞的能力与胞内外NAD浓度梯度正相关。该可逆透性化方法对生物催化和药物递送等研究具有重要价值。An efficient,reversible cell permeabilization method was developed by treatment of cells with sepiolite nanofibers.When the suspension of sepiolite nanofibers and Escherichia coli BW25113 cells were votexed,90% of the cells were found susceptible to bleomycin,whereas only 23% of the cells were susceptible upon proper resealing at 4 ℃.Nicotinamide adenosine dinucleotide（NAD） was either released or taken up by permeabilized cells,and the direction and rate of NAD diffusion were positively correlated to the concentration gradients across the cell membrane.This cell permeabilization method should be useful for biocatalysis and drug delivery.

关 键 词：细胞透性化 海泡石 博莱霉素 烟酰胺腺嘌呤二核苷酸 矿石纳米材料 

分 类 号：Q819[生物学—生物工程]