湖光岩玛珥湖浮游植物、浮游细菌生物量与可培养细菌数量的周年变化  

Annual Dynamics of Phytoplankon Biomass, Bacterioplankton Biomass and Densities of Culturable Bacteria in Huguangyan Maar Lake

作　　者：贺艳艳[1] 李琴[1] 张瑜斌[1] 

机构地区：[1]广东海洋大学海洋资源与环境监测中心,广东湛江524088

出　　处：《广东海洋大学学报》2015年第1期62-68,共7页Journal of Guangdong Ocean University

基　　金：广东省科技计划项目(2013B020309011);广东省教育厅科技创新项目(2013KJCX0095)

摘　　要：采用丙酮萃取分光光度法、荧光显微计数法、平板计数法分别研究湖光岩玛珥湖5个站位表层水体浮游植物生物量、浮游细菌生物量、可培养细菌数量的周年变化。结果表明,湖光岩玛珥湖浮游植物生物量（以碳C计）和浮游细菌生物量（以C计）、可培养细菌数量周年波动范围分别为84.17~243.64 mg/m3、20.53~43.60 mg/m3、0.10×10^3~3.63×10^3 m L-1。三者的周年变化趋势呈规律性同步增减,峰值均出现在3月份,且在5个采样站位间差异无统计学意义（P〉0.05）。与主要生态因子的相关分析显示,浮游植物与浮游细菌生物量均与总氮呈显著正相关（P〈0.05）,而可培养细菌数量与氨氮呈显著正相关（P〈0.05）,与溶解性氮和磷酸盐呈显著负相关（P〈0.05）,但三者与多数调查的生态因子之间相关性无统计学意义（P〉0.05）。浮游植物的初级生产可能是调控细菌周年变化潜在的主导因素。The fluorescence microscopic enumeration, spectrophotometry method based on extraction with acetone and plate count method were respectively employed to survey the annual dynamics in phytoplankon biomass, bacterioplankton biomass and densities of culturable bacteria inhabiting in the surface water at five sampling stations in Huguangyan Maar Lake, Zhangjiang. The biomass of phytoplankton and bacterioplankton ranged from 84.17 mg/m3 to 243.64 mg/m3, and from 20.53 mg/m3 to 43.60 mg/m3, respectively. Nevertheless, the densities of culturable bacteria varied from 0.10×10^3 to 3.63×10^3 cfu/m L. The bacterioplankton biomass, phytoplankton biomass and densities of culturable bacteria revealed the synchronous increase or decline with the peak in March within one year. No significant differences in three investigated objectives were found among 5 sampling stations（P〉0.05）. Pearson correlation coefficients showed the significantly positive correlations between the biomass of bacterioplankton and phytoplankton and total nitrogen（TN）（P〈0.05）, while the densities of culturable bacteria was significantly positive correlative to NH3--N（P〈0.05）, and significantly negative correlative to dissoluble nitrogen（DN） and PO43--P（P〈0.05）. No significant correlations were discovered between the bacteria, phytoplankton and other selected ecological factors（P〉0.05）. The primary production from phytoplankton was perhaps a potential key factor regulating the annual variation of bacteria.

关 键 词：湖光岩玛珥湖 浮游植物 浮游细菌 可培养细菌 周年变化 

分 类 号：Q938.1[生物学—微生物学] Q948.8