刺血疗法对急性痛风性关节炎大鼠局部抗炎因子的影响  

Effects of Pricking Bloodletting Therapy on Local Anti-inflammatory Cytokine in Rats with Acute Gouty Arthritis on Ankle

作　　者：吕凯露[1] 夏有兵[1,2] 程洁[1] 穆艳云[1] 朱冰梅[1] 罗玺[2] 梁莎 庄克清 

机构地区：[1]南京中医药大学,江苏南京市210023 [2]南京医科大学,江苏南京市210029 [3]湖南医药学院,湖南怀化市418000

出　　处：《中国康复理论与实践》2015年第3期276-279,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(No.81273840)

摘　　要：目的探讨刺血疗法治疗急性痛风性关节炎的相关抗炎分子机制。方法 60只Sprague-Dawley大鼠等分为正常组、正常刺血组、假手术组、模型组、刺血组、药物组。右踝关节腔注射尿酸钠建立急性痛风性关节炎大鼠模型。药物组以布洛芬灌胃治疗,正常刺血组和刺血组在右侧昆仑穴点刺放血。治疗前后测量各组大鼠踝关节周径。用RT-PCR法和Western blotting法检测大鼠踝关节白细胞介素(IL)-10、IL-4 m RNA和蛋白表达。结果治疗后刺血组较正常组踝关节周径增大(P<0.05),较模型组、药物组周径减小(P<0.05)。刺血组较正常组、模型组、药物组踝关节IL-10 m RNA的相对表达量升高(P<0.05),较正常组、模型组IL-10蛋白表达增加(P<0.05);刺血组踝关节IL-4 m RNA和蛋白表达较正常组有升高趋势,但无显著性差异(P>0.05)。结论刺血疗法通过促进局部IL-10表达发挥抗炎作用,可能是其治疗急性痛风性关节炎的作用机制之一。Objective To explore the anti- inflammatory of pricking blood therapy in acute gouty arthritis rats. Methods 60Sprague- Dawley rats were equally divided into normal group, bloodletting normal group, sham group, arthritis group, bloodletting group and ibuprofen group. The acute gouty arthritis model was established with injecting uric acid sodium salt into the right ankle joint cavity.The ibuprofen group was administrated with ibuprofen intragastrically, the bloodletting normal group and bloodletting group were pricked the right Kunlun（BL60） acupoint. The cross section diameter of the right ankle joint were measured before and after treatment. Levels of m RNA and protein of interleukin（IL）-10 and IL-4 expressed in ankle were determined with RT-PCR and Western blotting Results The cross section diameter increased in the bloodletting group compared with the normal group after treatment（P〈0.05）, and decreased（P〈0.05） compared with the arthritis group and the ibuprofen group, while the expression of IL-10 m RNA increased（P〈0.05） compared with the normal group, the arthritis group and the ibuprofen group, as well as the IL-10 protein compared with the normal group and the arthritis group（P〈0.05）. The expression of IL-4 m RNA and protein increased without significance（P〉0.05） in the bloodletting group compared with the normal group. Conclusion IL-10 may play a role of anti-inflammatory in pricking bloodletting therapy for acute gouty arthritis.

关 键 词：急性痛风性关节炎 刺血疗法 白细胞介素-10 白细胞介素-4 炎症 

分 类 号：R589.7[医药卫生—内分泌]