额尔敦·乌日勒对大鼠脊髓损伤后Nogo-A的调节作用  

Middletown Amount Regulation Wurih Le After Spinal Cord Injury in The Nogo-A

作　　者：张建宇[1] 韩滨[2] 叶纪诚[1] 王海生[1] 邓秀玲[1] 苑红[1] 武瑞兵[1] 

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010059 [2]武警内蒙古总队医院心内科,内蒙古呼和浩特010031

出　　处：《内蒙古医学杂志》2015年第2期129-131,F0004,共4页Inner Mongolia Medical Journal

基　　金：内蒙古医科大学青年创新基金(YKD2013QNCX006);内蒙古医科大学博士启动基金项目(BSJJ201405)

摘　　要：目的观察蒙药额尔敦·乌日勒在大鼠脊髓不完全损伤后的神经修复作用,探讨其对NogoA表达的调节作用。方法把成年Wistar大鼠48只随机分为损伤组(A组)和治疗组(B组),每组24只。利用改良Allen's方法建立脊髓不完全损伤模型。损伤后用额尔敦·乌日勒对B组进行灌胃治疗。将A、B组分为3、7、14 d 3个时间点,每个时间点8只。分别在3个时间点对A、B组大鼠的运动功能进行BBB评分,评分完毕处死动物并取受损节段脊髓行免疫组化染色观察Nogo-A在脊髓组织中的分布情况,同时进行阳性细胞计数,并作比较。结果 A、B组大鼠的运动功能在损伤后都有不同程度的恢复,B组7 d和14 d的BBB评分明显高于A组对应时间点,差异有统计学意义(P<0.05)。Nogo-A蛋白在B组的表达量显著低于A组,差异有显著性(P<0.05)。结论大鼠脊髓损伤后Nogo-A表达明显升高,额尔敦·乌日勒具有抑制Nogo-A表达的作用。Objective To investigate the effects of mongolian medicine Eerdun Wurile on the nerve regen-eration in rats with acute incomplete spinal cord injury and it＇ s regulating effect on expression of Nogo - A.Method Rat model of spinal cord injury was established by modified Allen＇s method. Forty- eight adult Wis-tar rats were divided into the spinal cord injury group （group A, n = 24） and group B （n = 24） treated with Eerdun Wurile at random after spinal cord injury. The rats in group B was treated with Eerdun Wurile （1g/kg） once a day by gavage. At 3d, 7d and 14d after operation, motor functions of the rat hind limbs were determined by the BBB （Basso Beattie and Bresnahan）, and the rats in each group were killed at 3d, 7d and 14d post opera-tion for historygcial examination. Morphologic changes of spinal neurons were evaluated by HE staining and im-munohistochemistry, separately. Results It was observed that at 3d, 7d and 14d after operation, the motor functions of the rat hind limbs about the acute spinal cord injury in each group were recovered. The expression levels of Nogo - A were significantly decreased in group B（P 〈 0.05）. Conclusion The expression of Nogo - A was markedly increased in rats with acute spinal cord injury, and early use of Eerdun Wurile may inhibit the ex-pression of Nogo - A.

关 键 词：脊髓损伤 额尔敦·乌日勒 NOGO-A 

分 类 号：R971.9[医药卫生—药品]