HMC毒素处理诱导玉米幼苗根冠细胞凋亡的结果观察  

The Results Observe of Induced by HMC Toxin Treatment in Maize Seedling Root Cap Cell Apoptosis

作　　者：鲁长林 马春红[1] 及增发[3] 张红心[4] 赵璞[1] 李秀丽[1] 王立安[2] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北省植物转基因中心,河北石家庄050051 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北省农林科学院,河北石家庄050031 [4]唐山师范学院生命科学系,河北唐山063000

出　　处：《云南农业大学学报（自然科学版）》2015年第2期192-197,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：科技部科技伙伴计划(KY201402017);河北省自然科学基金(C2011301010);河北省高层次人才项目(A2010003028);河北省科技计划项目(13396401D);农业部"948"[2011-G1(4)-07]

摘　　要：以玉米小斑病菌C小种毒素(HMC-toxin)分别诱导2对同核异质体玉米的幼苗根冠细胞(即:基因型为B37的玉米C型不育细胞质CB37和同核保持系NB37,基因型为Mo17的玉米C型不育细胞质CMo17和同核保持系NMo17),采用吖啶橙(AO)与溴化乙啶(EB)荧光复染的方法,研究细胞发生凋亡的差异性及凋亡规律。结果表明:CB37的幼叶接种PBS再经150mg/L的HMC毒素处理后,其根冠细胞凋亡率达最高值为45.3%,而NB37仅为15.3%;CMo17凋亡率最高达63.7%,NMo17仅为6.39%;C细胞质的细胞死亡率远高于N细胞质,凋亡率随诱导浓度和诱导时间的增加而增加。HMC毒素诱导根冠细胞发生凋亡,活细胞核发出绿色荧光,坏死细胞核则呈现橙红色荧光,且细胞核呈松散的颗粒状或新月状,凋亡细胞则发出黄绿色或绿色荧光,染色体固缩成新月状或颗粒状。To study the feature of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from two pair of homokaryon C and N inbred line of maize. Acridine orange （AO） and ethidium bromide （EB） staining methods were used to detach the apoptosis of root cap cells. In the process of induction, root cap cells appeared apoptotic bodies and marginate of chromosome morphology. Living, necrosis and apoptosis cells could be distinguished clearly by AO and EB complex staining. The result showed that the ratio of apoptosis in C cells was sharply higher than thatof N, and the max ratios of apoptosis of B37-C and B37-N stained by AO and EB were 45.3% and 15.3%, respectively. In contrast, the max ratios of apoptosis of Mol7-C and Mol7~N stained by AO and EB were 63.7% and 6. 39%, respectively. It is concluded that harmed by HMC toxin, the root cap cells appear apoptosis. The ratio of apoptosis in C cytoplasm was higher than in N cytoplasm. Along with the increase of toxin concentration and treating time, the ratio of apoptosis became higher HMC toxin induced root cap cells apoptosis, cell nucleus issued a green fluorescent, necrotic nuclei are orange red fluorescence, and loose granular cell nucleus or crescent, apoptotic cells emit yellow or green fluorescence, chromosome becomes pyknotic crescent or granule shape.

关 键 词：玉米小斑病菌C小种 毒素 细胞凋亡 吖啶橙 溴化乙啶 

分 类 号：S513.034[农业科学—作物学]