科玛嘉显色培养基快速诊断生殖器念珠菌病3507例  

Rapid diagnosis for 3 570 cases with genital candidiasis by CHROMagar

作　　者：徐晓容[1] 李佳玟[1] 赖爱民 庄奕宏[3] 

机构地区：[1]汕头大学医学院第一附属医院皮肤科、汕头市潮南民生医院皮肤科,广东省汕头515041 [2]汕头市潮南民生医院皮肤科 [3]汕头大学医学院第一附属医院检验科

出　　处：《中国基层医药》2015年第5期673-675,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省社会发展领域科技计划项目（2010145）

摘　　要：目的：使用科玛嘉显色培养基（ CHROMagar ）对生殖器念珠菌病的致病菌进行快速诊断，并进行耐药性分析。方法采用直接镜检初检以及科玛嘉显色培养基菌种鉴定的方法对广东省汕头地区3507例生殖器念珠菌病患者进行致病菌研究，并对科玛嘉显色培养基鉴定阳性的2172株念珠菌进行药敏分析。结果 3507份标本直接镜检阳性率38．0％（1332／3507），科玛嘉显色培养基共鉴定出念珠菌2172株，检出率61．9％（2172／3507），其中白色念珠菌占82．2％（1786／2172），女性阴道分泌物中非白色念珠菌比例为26．0％（350／1347）。 2172株念珠菌均未表现出对两性霉素B的耐药性；对制霉菌素、唑类抗真菌药均出现不同程度的耐药。结论科玛嘉显色培养基可快速诊断生殖器念珠菌病；广东省汕头地区生殖器念珠菌病主要由白色念珠菌引起，非白念珠菌所致的念珠菌性外阴阴道炎感染的比例增加；临床念珠菌对唑类药物出现了不同程度的耐药性。Objective To identify the pathogen of genital candidiasis by CHROMagar and analyze the resist-ance status of antifungals .Methods Administer direct microscopy examination and CHROMagar culture after sam-pling from 3 507 cases of genital candidiasis; then proceed the in vitro sensitivity analysis of antifungals for 2 172 strains of Candida .Results Hyphae and spores were detected from 1 332 cases （ 1 332/3 507 ） .2 172 strains of Candida were isolated by CHROMagar culture （2 172/3 507 ） ,in which there were 1 786 strains of Candida albicans （1 786/2 172） ,while 350 strains of non -Candida albicans in female vaginal discharge （350/1 347）. In vitro sensi- tivity analysis of the antifungal drugs showed none resistance to amphotericin B and different degrees of resistance to azole antifungals and nystatin. Conclusion CHROMagar culture is a useful and rapid diagnostic method for genital candidiasis. Genital candidiasis in local area is mainly caused by Candida albicans ,while there were increasing portion of vulvovaginal candidiasis caused by non - Candida albicans. In vitro sensitivity analysis of the antifungal drugs showed different degrees of resistance to azole antifungals.

关 键 词：念珠菌病 科玛嘉显色培养基 药物耐受性 

分 类 号：R756.5[医药卫生—皮肤病学与性病学]