高效液相色谱法同时测定反应液中的苯并噻唑和2-巯基苯并噻唑  

The Simultaneous Determination of Benzothiazole and 2-mercaptobenzothiazole in the Reaction Liquid by High Performance Liquid Chromatography

作　　者：赵建宏[1] 张梅梅[1] 程相林[1] 王建设[1] 王留成[1] 宋成盈[1] 张家丽[1] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001

出　　处：《郑州大学学报（工学版）》2015年第1期37-40,共4页Journal of Zhengzhou University（Engineering Science）

基　　金：河南省产学研计划资助项目(142107000007)

摘　　要：建立了一种同时定量测定反应液中苯并噻唑和2-巯基苯并噻唑的高效液相色谱分析方法.该方法采用Hypersil C18（150 mm×4.6 mm i.d）型色谱柱,以V（甲醇）：V（水）=50∶50作为流动相（用氨水调节流动相的p H为7.0~8.0）;检测波长254 nm;柱温30℃;流速0.9 m L/min.结果表明,待测物苯并噻唑和2-巯基苯并噻唑在上述色谱条件下分离良好,两种物质的质量浓度线性范围分别为0.047~0.426 g·L^-1和0.059~0.273 g·L^-1,检出限分别在1.23×10^-3g·L^-1和0.78×10^-3g·L^-1,加标回收率分别在98.20%~101.03%和98.78%~99.76%,测定结果的最大相对标准偏差分别为0.45%和0.63%.A method was presented for the simultaneous determination of benzothiazole and 2-mercaptobenzothiazole in the reaction liquid by high performance liquid chromatography（ HPLC）. The analysis was performed on a Hypersil C18 column（ 150 mm × 4. 6 mm i. d） with 50： 50 methanol-water as the mobile phase（ mobile phase with ammonia,p H = 7. 0 ~ 8. 0） at a flow rate of 0. 9 m L / min. The samples were detected at 254 nm with an UV detector and the column temperature was 30 ℃. It was found that benzothiazole and 2-mercaptobenzothiazole could be well separated under the chromatographic conditions. The linear ranges of the mass concentration were 0. 047 ~ 0. 426 g / L and 0. 059 g / L ~ 0. 273 g / L respectively. The recoveries for them were98. 20% ~ 101. 0 3% and 98. 78 % ~ 99. 76%. The maximum relative standard deviations were 0. 45 % and0. 63 % for benzothiazole and 2-mercaptobenzothiazole.

关 键 词：高效液相色谱 2-巯基苯并噻唑 苯并噻唑 

分 类 号：O657.7[理学—分析化学]