双氢麦角碱对急性酒精中毒大鼠海马DG区氨基酸含量的影响  

The effect of dihydroergotoxine on amino acids levels in hippocampus DG of rats with acute alcoholism

作　　者：许银花[1] 吴光[1] 郑雪梅[1] 崔松彪[1] 

机构地区：[1]延边大学附属医院神经内科,吉林延吉133000

出　　处：《中风与神经疾病杂志》2015年第3期239-242,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的观察双氢麦角碱对急性酒精中毒大鼠海马齿状回(dentate gyrus,DG)区细胞外液部分氨基酸含量的影响。方法将成年Wistar大鼠(270±30 g)18只随机分成对照组(n=6)、酒精组(n=6)和治疗组(n=6)。酒精组与治疗组大鼠胃内先灌入50%食用酒精,每次0.5 ml,每10 min灌1次,直到大鼠出现酒精中毒状态为止。30 min后,治疗组大鼠灌胃双氢麦角碱0.7 mg/kg,酒精组灌胃同等剂量的生理盐水,对照组给予同等剂量的生理盐水。各组动物用脑内微量透析法及高效液相色谱法测定海马DG区细胞外液中的几种氨基酸类神经递质的含量,包括谷氨酸(glutamate,Glu)、天冬氨酸(aspartate,Asp)、谷氨酰胺(Glutamine,Gln)、甘氨酸(Glycine,Gly)、牛黄酸(Taurine,Tau)和丙氨酸(Alanine,Ala)。结果 (1)与对照组相比,急性酒精中毒时大鼠海马DG区细胞外液中的Asp、Glu和Gly的含量均明显增加(均为P<0.05),而Gln、Tau和Ala含量无明显变化(均为P>0.05)。(2)治疗组大鼠海马DG区细胞外液中的Asp、Glu和Gly的含量与对照组相比无显著差异(均为P>0.05)。结论(1)急性酒精中毒与海马DG区Asp、Glu和Gly含量增加有关。(2)双氢麦角碱可降低急性酒精中毒导致的大鼠海马DG区Asp、Glu和Gly含量。Objective To investigate the effect of dihydroergotoxine on amino acids levels in hippocampus DG of rats with acute alcoholism. Methods Eighteen three-month-old rats were randomized into control group（ n = 6） 、alcohol group（ n = 6） and treatment group（ n = 6）. Alcohol group and treatment group rats were orally exposed to 50% ethanol0. 5 ml per 10 minute,untill the rat to the alcoholics condition. After 30 minutes,D treatment group were orally exposed to dihydroergotoxine（ 0. 7 mg / kg） and Alcohol group were orally exposed to the same dose of saline. Control group were orally exposed to the same dose of saline. The microdialysis technique and highperformance liquid chromatography was used to quantify Glutamate（ Glu） 、Aspartate（ Asp） 、Glutamine（ Gln） 、Glycine（ Gly） 、Taurine（ Tau） and Alanine（ Ala） in hippocampus DG. Results（ 1） Theglutamate、asptamate and Glycine in ethanol group were significantly increased than that in Control group（ P〈0. 05,respectively）.（ 2） Concerning glutamate、asptamate and glycine,there was no significant differrnce between treatment and control group（ P〉0. 05,respectively）. Conclusion（ 1） Acute alcoholism is accompanied with Glu、Asp and Gly release in hippocampus DG.（ 2） Dihydroergotoxine can reduce acute alcoholism syndromes,which may be related with decreasing Glu、Asp、Gly acid levels in hippocampus DG.

关 键 词：酒精中毒 氨基酸类 海马齿状回区 脑部微量透析 二氢麦角碱 

分 类 号：R595.6[医药卫生—内科学]