表面增强拉曼散射探测细胞酶的活性  

Detection of Cellular Enzymatic Activity by Enhanced Raman Scattering

作　　者：雷芸[1] 徐骏[1] 

机构地区：[1]武汉理工大学资源与环境工程学院,武汉430070

出　　处：《光散射学报》2015年第1期35-38,共4页The Journal of Light Scattering

基　　金：高等学校博士学科点专项科研基金资助课题(20100143120012);国家自然科学基金资助项目(51204129)

摘　　要：本文以5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷为底物,探测细胞结构中β-半乳糖苷酶的活性。作为β-半乳糖苷酶的底物,5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷在细胞β-半乳糖苷酶酶解作用下,形成靛蓝染料衍生物,在贵金属表面具有较强的表面增强拉曼散射。这种通过表面增强拉曼光谱探测细胞酶活性的方法,与高速拉曼线扫描相结合,可以快速地评估细胞种群的酶活性。The activity ofβ-galactosidase in cellular substructures has been identified by surface enhanced Raman scattering（SERS）.The new approach is based on the 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside（X-gal）as a substrate of β-galactosidase.The enzymes in cells hydrolysis ofβ-galactosidase led to the formation of indigo dye derivatives,which were found to be highly SERS active on the surface of noble metal.Combining the method with high speed Raman line mapping allows enzymatic activity to be assessed rapidly and potentially over large cell populations.

关 键 词：表面增强拉曼散射 Β-半乳糖苷酶 拉曼线扫描 

分 类 号：O657.37[理学—分析化学]