衍生于双氰基二苯代乙烯的用于活体成像的双光子荧光锌离子探针  

Dicyanostilbene-derived Two-photon Fluorescence Probe for Free Zinc Ions in Live Cells and Living Tissues

作　　者：黄池宝[1,2] 梁兴[1] 曾启华[1] 陈华仕[1] 曾伯平[1] 易道生[2] 陈晓远[2] 

机构地区：[1]遵义师范学院化学化工学院,遵义563002 [2]韶关学院英东农业科学与工程学院,韶关512005

出　　处：《高等学校化学学报》2015年第4期646-653,共8页Chemical Journal of Chinese Universities

基　　金：中国博士后科学基金(批准号:20100471224);广东省自然科学基金(批准号:s2011040000536);湖南省科技计划项目(批准号:2011RS4020);深圳大学省部重点实验室开放基金项目(批准号:201202);韶关市科技计划项目(批准号:2011CX/K19);贵州省教育厅自然科学重点研究项目(批准号:黔教合KY字[2014]296)~~

摘　　要：制备了衍生于双氰基二苯代乙烯的双光子荧光锌离子探针,该探针以4-（2-吡啶甲基）哌嗪为锌离子受体,当络合锌离子时,探针的荧光强度增强了72.5倍和580 GM的双光子吸收截面.该探针无细胞毒性且在体内环境中无p H敏感性,其解离常数KTPd=（0.52±0.01）μmol/L.性能测试结果表明,该探针能选择性地检测活细胞中的游离锌离子,耐时长达约1500 s,且能探测活体组织80~150μm深处的锌离子,不受其它金属离子与生物膜的干扰.A novel two-photon fluorescence probe for Zn2＋derived from dicyanostilbene as a two-photon fluorophore and 4-（ pyridine-2-ylmethyl） piperazine as a novel Zn2＋ligand was developed,and its structure was characterized by1 H NMR,IR and elemental analysis. By UV-Vis absorption and absorption-titration experiments,one- and two-photon excited fluorescence experiments in femtosecond pulses,as well as cell-imaging and tissue-imaging experiments,the probe showed a 72. 5-fold fluorescence enhancement in response to Zn2＋,a large two-photon action cross-section（ 580 GM）,a noncytotoxic effect,and p H insensitivity in the biologically relevant range,with a dissociation constant（ KTPd） of（ 0. 52±0. 01） μmol/L. The probe could detect free Zn2＋ions selectively in live cells for about 1500 s and in living tissues at a depth of 80—150 μm without interference from other metal ions or membrane-bound probes.

关 键 词：双光子荧光探针 锌离子 活体成像 双光子发射截面 

分 类 号：O655.25[理学—分析化学]