呋喃妥因抗原的制备及其在免疫胶体金快速检测中的应用  

Preparation of Nitrofurantoin Metabolite Antigen and its Application of Rapid Detection of Nitrofurantoin Metabolite Residue by Colloidal Goad-based Immunochromatography

作　　者：潘嘉慧[1] 田峻[1] 沈国权[1] 赵亮亮[1] 朱海云[1] 

机构地区：[1]佛山市质量计量监督检测中心,广东佛山528225

出　　处：《广东化工》2015年第5期34-35,共2页Guangdong Chemical Industry

基　　金：佛山市科技发展专项资金项目【2012AA100591】

摘　　要：本研究从优化呋喃妥因代谢物免疫抗原、包被抗原的结构及优化偶联技术角度出发,提高呋喃妥因代谢物抗体的特异性、亲和力,并通过优化样品前处理步骤,最终得到呋喃妥因代谢物胶体金免疫层析试纸条的检测低限为1.0μg/kg,特异性好,稳定性高。The study optimized the structure of nitrofurantoin metabolite immune antigen,envelope antigen and the coupling technology,improving the specificity of antibody of nitrofurantoin metabolite,affinity,and simplifying the sample preparation procedures.The results showed that the rapid detection limit of nitrofurantoin metabolite residue by colloidal goad-based immunochromatography can reach 1.0μg/kg,showing good specificity and high stability.

关 键 词：呋喃妥因代谢物抗原 免疫胶体金 快速检测 

分 类 号：TQ[化学工程]