树舌多糖GF对小鼠HepA瘤Rb基因表达的影响  

Effect of Ganoderma appanatum polysaccharides GF on Rb gene expression in mouse HepA tumour

作　　者：张庆梅[1] 刘丽波[1] 潘洪明[1] 于英君[1] 

机构地区：[1]黑龙江中医药大学生化与分子生物学教研室,黑龙江哈尔滨150040

出　　处：《中医药学刊》2002年第4期431-431,443,共2页Study Journal of Traditional Chinese Medicine

基　　金：黑龙江省科技攻关项目

摘　　要：为探明树舌多糖GF对小鼠HepA瘤Rb基因表达的影响。本实验用链霉菌抗生素蛋白 -过氧化酶免疫组化法来测定瘤组织中Rb蛋白含量 ,通过多媒体图像分析系统分析实验结果。结果表明 :树舌多糖GF组、猪苓多糖组与荷瘤对照组比较 ,差异均非常显著 (P <0 .0 1) ,即树舌多糖GF、猪苓多糖明显增强HepA瘤组织中Rb基因的表达 ;树舌多糖组与猪苓多糖组比较 ,差异非常显著 (P <0 .0 1) ,即树舌多糖GF更能显著增强Rb基因表达 ,优于猪苓多糖。因此可初步认为 ,作用于抑癌基因Rb并使之表达增强 ,是树舌多糖GF抗瘤作用机制之一。Objective:To study the effect of GAPSGF on Rb gene expression in HepA tumour.Mettods:The level of Rb gene expression is determined by UltraSensitive TM S-P Kit.the kit is based on labeled Streptavidin-Peroxidase immuno-histochemistry method.the ultimate results are gotten by mutimedia colorful pathologic image processing system.Results:The average levels of Rb gene expression in GAPSGF group and PUPS group have much differecnce with control group.that is to say,GAPSGF and PUPS can significantly strengthen the expression of Rb gene in HepA cells.GAPSGF can more remarkably improve the levels of Rb gene expression than PUPS.Conclusion:Gapsgf effect on Rb expression is one of the mechanisms of antitumor activity.

关 键 词：树舌多糖GF 猪苓多糖 HepA肿瘤组织 肝组织 链霉菌抗生素蛋白-过氧化酶免疫组化法 多媒体彩色病理图文分析系统 

分 类 号：R285.5[医药卫生—中药学] R273[医药卫生—中医学]